1hkz

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==About this Structure==
==About this Structure==
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1HKZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with CU1, CU, C1O and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HKZ OCA]].
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1HKZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with CU1, CU, C1O and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HKZ OCA].
==Reference==
==Reference==
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[[Category: sporulation]]
[[Category: sporulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:38:08 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:46:33 2007''

Revision as of 10:41, 5 November 2007

Image:1hkz.gif

1hkz, resolution 2.49Å

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CRYSTAL STRUCTURE OF BACILLUS SUBTILIS COTA AFTER 8H SOAKING WITH ABTS

Overview

The CotA laccase from the endospore coat of Bacillus subtilis has been, crystallized in the presence of the non-catalytic co-oxidant, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and the, structure was determined using synchrotron radiation. The binding site for, this adduct is well defined and indicates how ABTS, in conjunction with, laccases, could act as an oxidative mediator toward non-phenolic moieties., In addition, a dioxygen moiety is clearly defined within the solvent, channel oriented toward one of the T3 copper atoms in the trinuclear, center.

About this Structure

1HKZ is a Single protein structure of sequence from Bacillus subtilis with CU1, CU, C1O and GOL as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.

Reference

Substrate and dioxygen binding to the endospore coat laccase from Bacillus subtilis., Enguita FJ, Marcal D, Martins LO, Grenha R, Henriques AO, Lindley PF, Carrondo MA, J Biol Chem. 2004 May 28;279(22):23472-6. Epub 2004 Feb 4. PMID:14764581

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