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3epv
From Proteopedia
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| - | [[ | + | ==X-ray Structure of the Metal-sensor CnrX in both the Apo- and Copper-bound Forms== |
| + | <StructureSection load='3epv' size='340' side='right' caption='[[3epv]], [[Resolution|resolution]] 1.74Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3epv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupriavidus_metallidurans Cupriavidus metallidurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EPV FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cnrR, cnrX, RMe0087, Rmet_6206 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=119219 Cupriavidus metallidurans])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3epv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3epv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3epv RCSB], [http://www.ebi.ac.uk/pdbsum/3epv PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ep/3epv_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Both the X-ray structures of the apo- and the copper-bound forms of the metal-sensor domain (residues 31-148) of CnrX from Cupriavidus metallidurans CH34 were obtained at 1.74A resolution from a selenomethionine derivative. This four-helix hooked-hairpin is the first structure of a metal-sensor in an ECF-type signaling pathway. The copper ion is bound in a type 2-like center with a 3N1O coordination in the equatorial plane and shows an unprecedented remote fifth axial ligand with Met93 contributing a weak S-Cu bond. The signal onset cannot be explained by conformational changes associated with CnrX metallation. | ||
| - | + | X-ray structure of the metal-sensor CnrX in both the apo- and copper-bound forms.,Pompidor G, Maillard AP, Girard E, Gambarelli S, Kahn R, Coves J FEBS Lett. 2008 Nov 26;582(28):3954-8. Epub 2008 Nov 4. PMID:18992246<ref>PMID:18992246</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Cupriavidus metallidurans]] | [[Category: Cupriavidus metallidurans]] | ||
| - | [[Category: Coves, J | + | [[Category: Coves, J]] |
| - | [[Category: Gambarelli, S | + | [[Category: Gambarelli, S]] |
| - | [[Category: Girard, E | + | [[Category: Girard, E]] |
| - | [[Category: Kahn, R | + | [[Category: Kahn, R]] |
| - | [[Category: Maillard, A P | + | [[Category: Maillard, A P]] |
| - | [[Category: Pompidor, G | + | [[Category: Pompidor, G]] |
[[Category: All alpha helix]] | [[Category: All alpha helix]] | ||
[[Category: Cobalt]] | [[Category: Cobalt]] | ||
[[Category: Metal binding protein]] | [[Category: Metal binding protein]] | ||
[[Category: Nickel]] | [[Category: Nickel]] | ||
Revision as of 08:55, 26 November 2014
X-ray Structure of the Metal-sensor CnrX in both the Apo- and Copper-bound Forms
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