4hx9
From Proteopedia
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| - | [[ | + | ==Designed Phosphodeoxyribosyltransferase== |
| + | <StructureSection load='4hx9' size='340' side='right' caption='[[4hx9]], [[Resolution|resolution]] 2.68Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4hx9]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_leichmanni_i"_(sic)_henneberg_1903 "bacillus leichmanni i" (sic) henneberg 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HX9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ntd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28039 "Bacillus Leichmanni I" (sic) Henneberg 1903])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside_deoxyribosyltransferase Nucleoside deoxyribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.6 2.4.2.6] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hx9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hx9 RCSB], [http://www.ebi.ac.uk/pdbsum/4hx9 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A large number of nucleoside analogs and 2'- deoxynucleoside triphosphates (dNTPs) have been synthesized in order to interfere with DNA metabolism. However, in vivo the concentration and phosphorylation of these analogues are key limiting factors. In this context, we designed enzymes to switch nucleobases attached to a deoxyribose monophosphate. Active chimeras were made from two distantly related enzymes: a nucleoside deoxyribosyltransferase (NDT) from lactobacilli and a 5'-monophosphate-2'-deoxyribonucleoside hydrolase (Rcl) from rat. Then, their unprecedented activity was further extended to deoxyribose triphosphate and, in vitro biosyntheses could be successfully performed with several base analogues. These new enzymes provide new tools to synthesize dNTPs analogs and to deliver them into cells. | ||
| - | + | Phosphodeoxyribosyltransferases: designed enzymes for deoxyribonucleotide synthesis.,Kaminski PA, Labesse G J Biol Chem. 2013 Jan 16. PMID:23325804<ref>PMID:23325804</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
| - | + | ||
[[Category: Nucleoside deoxyribosyltransferase]] | [[Category: Nucleoside deoxyribosyltransferase]] | ||
| - | [[Category: Kaminski, P A | + | [[Category: Kaminski, P A]] |
| - | [[Category: Labesse, G | + | [[Category: Labesse, G]] |
[[Category: Non natural and design enzyme]] | [[Category: Non natural and design enzyme]] | ||
[[Category: Phosphodeoxyribosyltransferase]] | [[Category: Phosphodeoxyribosyltransferase]] | ||
[[Category: Rossmann fold]] | [[Category: Rossmann fold]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 17:04, 10 December 2014
Designed Phosphodeoxyribosyltransferase
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