1zwx
From Proteopedia
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| - | [[Image:1zwx.gif|left|200px]] | + | [[Image:1zwx.gif|left|200px]] |
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| - | '''Crystal Structure of SmcL''' | + | {{Structure |
| + | |PDB= 1zwx |SIZE=350|CAPTION= <scene name='initialview01'>1zwx</scene>, resolution 1.900Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Sphingomyelin_phosphodiesterase Sphingomyelin phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.12 3.1.4.12] | ||
| + | |GENE= smcl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1638 Listeria ivanovii]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of SmcL''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZWX is a [ | + | 1ZWX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Listeria_ivanovii Listeria ivanovii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZWX OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of SmcL, a bacterial neutral sphingomyelinase C from Listeria., Openshaw AE, Race PR, Monzo HJ, Vazquez-Boland JA, Banfield MJ, J Biol Chem. 2005 Oct 14;280(41):35011-7. Epub 2005 Aug 10. PMID:[http:// | + | Crystal structure of SmcL, a bacterial neutral sphingomyelinase C from Listeria., Openshaw AE, Race PR, Monzo HJ, Vazquez-Boland JA, Banfield MJ, J Biol Chem. 2005 Oct 14;280(41):35011-7. Epub 2005 Aug 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16093240 16093240] |
[[Category: Listeria ivanovii]] | [[Category: Listeria ivanovii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: dnase1-like fold]] | [[Category: dnase1-like fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:40:49 2008'' |
Revision as of 13:40, 20 March 2008
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| , resolution 1.900Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | smcl (Listeria ivanovii) | ||||||
| Activity: | Sphingomyelin phosphodiesterase, with EC number 3.1.4.12 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of SmcL
Overview
Sphingomyelinases C are enzymes that catalyze the hydrolysis of sphingomyelin in biological membranes to ceramide and phosphorylcholine. Various pathogenic bacteria produce secreted neutral sphingomyelinases C that act as membrane-damaging virulence factors. Mammalian neutral sphingomyelinases C, which display sequence homology to the bacterial enzymes, are involved in sphingolipid metabolism and signaling. This article describes the first structure to be determined for a member of the neutral sphingomyelinase C family, SmcL, from the intracellular bacterial pathogen Listeria ivanovii. The structure has been refined to 1.9-A resolution with phases derived by single isomorphous replacement with anomalous scattering techniques from a single iridium derivative. SmcL adopts a DNase I-like fold, and is the first member of this protein superfamily to have its structure determined that acts as a phospholipase. The structure reveals several unique features that adapt the protein to its phospholipid substrate. These include large hydrophobic beta-hairpin and hydrophobic loops surrounding the active site that may bind and penetrate the lipid bilayer to position sphingomyelin in a catalytically competent position. The structure also provides insight into the proposed general base/acid catalytic mechanism, in which His-325 and His-185 play key roles.
About this Structure
1ZWX is a Single protein structure of sequence from Listeria ivanovii. Full crystallographic information is available from OCA.
Reference
Crystal structure of SmcL, a bacterial neutral sphingomyelinase C from Listeria., Openshaw AE, Race PR, Monzo HJ, Vazquez-Boland JA, Banfield MJ, J Biol Chem. 2005 Oct 14;280(41):35011-7. Epub 2005 Aug 10. PMID:16093240
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