1zxi

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[[Image:1zxi.gif|left|200px]]<br /><applet load="1zxi" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1zxi.gif|left|200px]]
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caption="1zxi, resolution 1.7&Aring;" />
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'''Reconstituted CO dehydrogenase from Oligotropha carboxidovorans'''<br />
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{{Structure
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|PDB= 1zxi |SIZE=350|CAPTION= <scene name='initialview01'>1zxi</scene>, resolution 1.7&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=CUM:CU(I)-S-MO(VI)(=O)OH+CLUSTER'>CUM</scene>, <scene name='pdbligand=MCN:PTERIN+CYTOSINE+DINUCLEOTIDE'>MCN</scene> and <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Carbon-monoxide_dehydrogenase_(acceptor) Carbon-monoxide dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2]
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|GENE=
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}}
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'''Reconstituted CO dehydrogenase from Oligotropha carboxidovorans'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1ZXI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Oligotropha_carboxidovorans Oligotropha carboxidovorans] with <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=FES:'>FES</scene>, <scene name='pdbligand=CUM:'>CUM</scene>, <scene name='pdbligand=MCN:'>MCN</scene> and <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbon-monoxide_dehydrogenase_(acceptor) Carbon-monoxide dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.99.2 1.2.99.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZXI OCA].
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1ZXI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Oligotropha_carboxidovorans Oligotropha carboxidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZXI OCA].
==Reference==
==Reference==
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Structural and functional reconstruction in situ of the [CuSMoO2] active site of carbon monoxide dehydrogenase from the carbon monoxide oxidizing eubacterium Oligotropha carboxidovorans., Resch M, Dobbek H, Meyer O, J Biol Inorg Chem. 2005 Aug;10(5):518-28. Epub 2005 Sep 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16091936 16091936]
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Structural and functional reconstruction in situ of the [CuSMoO2] active site of carbon monoxide dehydrogenase from the carbon monoxide oxidizing eubacterium Oligotropha carboxidovorans., Resch M, Dobbek H, Meyer O, J Biol Inorg Chem. 2005 Aug;10(5):518-28. Epub 2005 Sep 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16091936 16091936]
[[Category: Carbon-monoxide dehydrogenase (acceptor)]]
[[Category: Carbon-monoxide dehydrogenase (acceptor)]]
[[Category: Oligotropha carboxidovorans]]
[[Category: Oligotropha carboxidovorans]]
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[[Category: molybdoprotein]]
[[Category: molybdoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:20:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:41:00 2008''

Revision as of 13:41, 20 March 2008

Image:1zxi.gif


PDB ID 1zxi

Drag the structure with the mouse to rotate
, resolution 1.7Å
Ligands: , , , , and
Activity: Carbon-monoxide dehydrogenase (acceptor), with EC number 1.2.99.2
Coordinates: save as pdb, mmCIF, xml



Reconstituted CO dehydrogenase from Oligotropha carboxidovorans


Overview

Carbon monoxide dehydrogenase from the bacterium Oligotropha carboxidovorans catalyzes the oxidation of CO to CO(2) at a unique [CuSMoO(2)] cluster. In the bacteria the cluster is assembled post-translational. The integration of S, and particularly of Cu, is rate limiting in vivo, which leads to CO dehydrogenase preparations containing the mature and fully functional enzyme along with forms of the enzyme deficient in one or both of these elements. The active sites of mature and immature forms of CO dehydrogenase were converted into a [MoO(3)] centre by treatment with potassium cyanide. We have established a method, which rescues 50% of the CO dehydrogenase activity by in vitro reconstitution of the active site through the supply of sulphide first and subsequently of Cu(I) under reducing conditions. Immature forms of CO dehydrogenase isolated from the bacterium, which were deficient in S and/or Cu at the active site, were similarly activated. X-ray crystallography and electron paramagnetic resonance spectroscopy indicated that the [CuSMoO(2)] cluster was properly reconstructed. However, reconstituted CO dehydrogenase contains mature along with immature forms. The chemical reactions of the reconstitution of CO dehydrogenase are summarized in a model, which assumes resulphuration of the Mo-ion at both equatorial positions at a 1:1 molar ratio. One equatorial Mo-S group reacts with Cu(I) in a productive fashion yielding a mature, functional [CuSMoO(2)] cluster. The other Mo-S group reacts with Cu(I), then Cu(2)S is released and an oxo group is introduced from water, yielding an inactive [MoO(3)] centre.

About this Structure

1ZXI is a Protein complex structure of sequences from Oligotropha carboxidovorans. Full crystallographic information is available from OCA.

Reference

Structural and functional reconstruction in situ of the [CuSMoO2] active site of carbon monoxide dehydrogenase from the carbon monoxide oxidizing eubacterium Oligotropha carboxidovorans., Resch M, Dobbek H, Meyer O, J Biol Inorg Chem. 2005 Aug;10(5):518-28. Epub 2005 Sep 23. PMID:16091936

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