1zzc

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[[Image:1zzc.gif|left|200px]]<br /><applet load="1zzc" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1zzc.gif|left|200px]]
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caption="1zzc, resolution 1.80&Aring;" />
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'''Crystal Structure of CoII HppE in Complex with Tris Buffer'''<br />
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{{Structure
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|PDB= 1zzc |SIZE=350|CAPTION= <scene name='initialview01'>1zzc</scene>, resolution 1.80&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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|ACTIVITY=
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|GENE= fom4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 Streptomyces wedmorensis])
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}}
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'''Crystal Structure of CoII HppE in Complex with Tris Buffer'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1ZZC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_wedmorensis Streptomyces wedmorensis] with <scene name='pdbligand=CO:'>CO</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZC OCA].
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1ZZC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_wedmorensis Streptomyces wedmorensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZC OCA].
==Reference==
==Reference==
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Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16015285 16015285]
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Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16015285 16015285]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptomyces wedmorensis]]
[[Category: Streptomyces wedmorensis]]
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[[Category: mononuclear iron enzyme]]
[[Category: mononuclear iron enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:20:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:41:37 2008''

Revision as of 13:41, 20 March 2008

Image:1zzc.gif


PDB ID 1zzc

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands: and
Gene: fom4 (Streptomyces wedmorensis)
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of CoII HppE in Complex with Tris Buffer


Overview

The biosynthetic pathway of the clinically important antibiotic fosfomycin uses enzymes that catalyse reactions without precedent in biology. Among these is hydroxypropylphosphonic acid epoxidase, which represents a new subfamily of non-haem mononuclear iron enzymes. Here we present six X-ray structures of this enzyme: the apoenzyme at 2.0 A resolution; a native Fe(II)-bound form at 2.4 A resolution; a tris(hydroxymethyl)aminomethane-Co(II)-enzyme complex structure at 1.8 A resolution; a substrate-Co(II)-enzyme complex structure at 2.5 A resolution; and two substrate-Fe(II)-enzyme complexes at 2.1 and 2.3 A resolution. These structural data lead us to suggest how this enzyme is able to recognize and respond to its substrate with a conformational change that protects the radical-based intermediates formed during catalysis. Comparisons with other family members suggest why substrate binding is able to prime iron for dioxygen binding in the absence of alpha-ketoglutarate (a co-substrate required by many mononuclear iron enzymes), and how the unique epoxidation reaction of hydroxypropylphosphonic acid epoxidase may occur.

About this Structure

1ZZC is a Single protein structure of sequence from Streptomyces wedmorensis. Full crystallographic information is available from OCA.

Reference

Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:16015285

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