1zz7
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1zz7.gif|left|200px]] | + | [[Image:1zz7.gif|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of FeII HppE in Complex with Substrate form 1''' | + | {{Structure |
| + | |PDB= 1zz7 |SIZE=350|CAPTION= <scene name='initialview01'>1zz7</scene>, resolution 2.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene> and <scene name='pdbligand=S0H:(S)-2-HYDROXYPROPYLPHOSPHONIC ACID'>S0H</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= fom4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 Streptomyces wedmorensis]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of FeII HppE in Complex with Substrate form 1''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1ZZ7 is a [ | + | 1ZZ7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_wedmorensis Streptomyces wedmorensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZ7 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:[http:// | + | Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16015285 16015285] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces wedmorensis]] | [[Category: Streptomyces wedmorensis]] | ||
| Line 24: | Line 33: | ||
[[Category: substrate-enzyme complex]] | [[Category: substrate-enzyme complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:41:35 2008'' |
Revision as of 13:41, 20 March 2008
| |||||||
| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | fom4 (Streptomyces wedmorensis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of FeII HppE in Complex with Substrate form 1
Overview
The biosynthetic pathway of the clinically important antibiotic fosfomycin uses enzymes that catalyse reactions without precedent in biology. Among these is hydroxypropylphosphonic acid epoxidase, which represents a new subfamily of non-haem mononuclear iron enzymes. Here we present six X-ray structures of this enzyme: the apoenzyme at 2.0 A resolution; a native Fe(II)-bound form at 2.4 A resolution; a tris(hydroxymethyl)aminomethane-Co(II)-enzyme complex structure at 1.8 A resolution; a substrate-Co(II)-enzyme complex structure at 2.5 A resolution; and two substrate-Fe(II)-enzyme complexes at 2.1 and 2.3 A resolution. These structural data lead us to suggest how this enzyme is able to recognize and respond to its substrate with a conformational change that protects the radical-based intermediates formed during catalysis. Comparisons with other family members suggest why substrate binding is able to prime iron for dioxygen binding in the absence of alpha-ketoglutarate (a co-substrate required by many mononuclear iron enzymes), and how the unique epoxidation reaction of hydroxypropylphosphonic acid epoxidase may occur.
About this Structure
1ZZ7 is a Single protein structure of sequence from Streptomyces wedmorensis. Full crystallographic information is available from OCA.
Reference
Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:16015285
Page seeded by OCA on Thu Mar 20 15:41:35 2008
