208d

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[[Image:208d.gif|left|200px]]<br /><applet load="208d" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:208d.gif|left|200px]]
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caption="208d, resolution 2.050&Aring;" />
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'''HIGH-RESOLUTION STRUCTURE OF A DNA HELIX FORMING (C.G)*G BASE TRIPLETS'''<br />
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{{Structure
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|PDB= 208d |SIZE=350|CAPTION= <scene name='initialview01'>208d</scene>, resolution 2.050&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''HIGH-RESOLUTION STRUCTURE OF A DNA HELIX FORMING (C.G)*G BASE TRIPLETS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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208D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=208D OCA].
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208D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=208D OCA].
==Reference==
==Reference==
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High-resolution structure of a DNA helix forming (C.G)*G base triplets., Van Meervelt L, Vlieghe D, Dautant A, Gallois B, Precigoux G, Kennard O, Nature. 1995 Apr 20;374(6524):742-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7715732 7715732]
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High-resolution structure of a DNA helix forming (C.G)*G base triplets., Van Meervelt L, Vlieghe D, Dautant A, Gallois B, Precigoux G, Kennard O, Nature. 1995 Apr 20;374(6524):742-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7715732 7715732]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Dautant, A.]]
[[Category: Dautant, A.]]
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[[Category: double helix]]
[[Category: double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:20:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:42:00 2008''

Revision as of 13:42, 20 March 2008


PDB ID 208d

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, resolution 2.050Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



HIGH-RESOLUTION STRUCTURE OF A DNA HELIX FORMING (C.G)*G BASE TRIPLETS


Overview

Triple helices result from interaction between single- and double-stranded nucleic acids. Their formation is a possible mechanism for recombination of homologous gene sequences in nature and provides, inter alia, a basis for artificial control of gene activity. Triple-helix motifs have been extensively studied by a variety of techniques, but few high-resolution structural data are available. The only triplet structures characterized so far by X-ray diffraction were in protein-DNA complexes studied at about 3 A resolution. We report here the X-ray analysis of a DNA nonamer, d(GCGAATTCG), to a resolution of 2.05 A, in which the extended crystal structure contains (C.G)*G triplets as a fragment of triple helix. The guanosine-containing chains are in a parallel orientation. This arrangement is a necessary feature of models for homologous recombination which results ultimately in replacement of one length of DNA by another of similar sequence. The present-structure agrees with many published predictions of triplex organization, and provides an accurate representation of an element that allows sequence-specific association between single- and double-stranded nucleic acids.

About this Structure

208D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

High-resolution structure of a DNA helix forming (C.G)*G base triplets., Van Meervelt L, Vlieghe D, Dautant A, Gallois B, Precigoux G, Kennard O, Nature. 1995 Apr 20;374(6524):742-4. PMID:7715732

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