232d
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:232d.gif|left|200px]] | + | [[Image:232d.gif|left|200px]] |
| - | + | ||
| - | '''THE HIGH RESOLUTION CRYSTAL STRUCTURE OF THE DNA DECAMER D(AGGCATGCCT)''' | + | {{Structure |
| + | |PDB= 232d |SIZE=350|CAPTION= <scene name='initialview01'>232d</scene>, resolution 1.300Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NCO:COBALT HEXAMMINE ION'>NCO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE HIGH RESOLUTION CRYSTAL STRUCTURE OF THE DNA DECAMER D(AGGCATGCCT)''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 232D is a [ | + | 232D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=232D OCA]. |
==Reference== | ==Reference== | ||
| - | The high resolution crystal structure of the DNA decamer d(AGGCATGCCT)., Nunn CM, Neidle S, J Mol Biol. 1996 Feb 23;256(2):340-51. PMID:[http:// | + | The high resolution crystal structure of the DNA decamer d(AGGCATGCCT)., Nunn CM, Neidle S, J Mol Biol. 1996 Feb 23;256(2):340-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8594201 8594201] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Neidle, S.]] | [[Category: Neidle, S.]] | ||
| Line 17: | Line 26: | ||
[[Category: a-dna]] | [[Category: a-dna]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
| - | [[Category: flipped-out | + | [[Category: flipped-out base]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:42:37 2008'' |
Revision as of 13:42, 20 March 2008
| |||||||
| , resolution 1.300Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE HIGH RESOLUTION CRYSTAL STRUCTURE OF THE DNA DECAMER D(AGGCATGCCT)
Overview
The crystal structure of the DNA decamer d(AGGCATGCCT) has been determined to a resolution of 1.3 A and R factor of 13.9%. The structure has a unique conformation with each of the decamer single strands forming base-pairing interactions with two symmetry-related strands. The central eight bases of the decamer form an A-DNA octamer duplex with one symmetry-related strand whilst the terminal 5'-A and T-3' bases are flipped out and away from the octamer helix axis to form base-pairing interactions with a second symmetry-related strand. These A.T base-pairs lie perpendicular to the crystallographic c axis and pack within the unit cell in conjunction with a symmetry-related A.T base-pair displaced by 3.4 A degrees along the c axis. A novel base triplet interaction of the type A*(G.C) is present in the structure with interaction from the major groove side of the terminal 5'-A base to the minor groove of the central A-DNA octamer. This structure reports the first example of cobalt hexammine binding to a right-handed DNA duplex. The crystallographic asymmetric unit contains two cobalt hexammine ligands with one site in the major groove coordinating via hydrogen bonds to the 5'-AGG bases, and the second site located between DNA molecules and interacting with the oxygen atoms of phosphate groups.
About this Structure
232D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The high resolution crystal structure of the DNA decamer d(AGGCATGCCT)., Nunn CM, Neidle S, J Mol Biol. 1996 Feb 23;256(2):340-51. PMID:8594201
Page seeded by OCA on Thu Mar 20 15:42:37 2008
