2cfm
From Proteopedia
(New page: 200px<br /> <applet load="2cfm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cfm, resolution 1.80Å" /> '''ATP-DEPENDENT DNA L...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2CFM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]] with MG and AMP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFM OCA]]. | + | 2CFM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]] with MG and AMP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Ligase Ligase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFM OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: protein-nucleotide complex]] | [[Category: protein-nucleotide complex]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:36:06 2007'' |
Revision as of 06:31, 30 October 2007
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ATP-DEPENDENT DNA LIGASE FROM PYROCOCCUS FURIOSUS
Overview
DNA ligases join single-strand breaks in double-stranded DNA, and are, essential to maintain genome integrity in DNA metabolism. Here, we report, the 1.8 A resolution structure of Pyrococcus furiosus DNA ligase (PfuLig), which represents the first full-length atomic view of an ATP-dependent, eukaryotic-type DNA ligase. The enzyme comprises the N-terminal, DNA-binding domain, the middle adenylation domain, and the C-terminal, OB-fold domain. The architecture of each domain resembles those of human, DNA ligase I, but the domain arrangements differ strikingly between the, two enzymes. The closed conformation of the two "catalytic core" domains, at the carboxyl terminus in PfuLig creates a small compartment, which, holds a non-covalently bound AMP molecule. This domain rearrangement, results ... [(full description)]
About this Structure
2CFM is a [Single protein] structure of sequence from [Pyrococcus furiosus] with MG and AMP as [ligands]. Active as [Ligase], with EC number [6.5.1.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The closed structure of an archaeal DNA ligase from Pyrococcus furiosus., Nishida H, Kiyonari S, Ishino Y, Morikawa K, J Mol Biol. 2006 Jul 28;360(5):956-67. Epub 2006 Jun 12. PMID:16820169
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