3fd9

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[[Image:3fd9.png|left|200px]]
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==Crystal Structure of the transcriptional anti-activator ExsD from Pseudomonas aeruginosa==
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<StructureSection load='3fd9' size='340' side='right' caption='[[3fd9]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3fd9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_ucbpp-pa14 Pseudomonas aeruginosa ucbpp-pa14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FD9 FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">exsD, PA14_42380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208963 Pseudomonas aeruginosa UCBPP-PA14])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fd9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fd9 RCSB], [http://www.ebi.ac.uk/pdbsum/3fd9 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fd/3fd9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The opportunistic pathogen P. aeruginosa utilizes a type III secretion system (T3SS) to support acute infections in predisposed individuals. In this bacterium, expression of all T3SS-related genes is dependent on the AraC-type transcriptional activator ExsA. Before host contact, the T3SS is inactive and ExsA is repressed by the antiactivator protein ExsD. The repression, thought to occur through direct interactions between the two proteins, is relieved upon opening of the type III secretion (T3S) channel when secretion chaperone ExsC sequesters ExsD. We have solved the crystal structure of Delta20ExsD, a protease-resistant fragment of ExsD that lacks only the 20 amino terminal residues of the wild-type protein at 2.6 A. Surprisingly the structure revealed similarities between ExsD and the DNA binding domain of transcriptional repressor KorB. A model of an ExsD-DNA complex constructed on the basis of this homology produced a realistic complex that is supported by the prevalence of conserved residues in the putative DNA binding site and the results of differential scanning fluorimetry studies. Our findings challenge the currently held model that ExsD solely acts through interactions with ExsA and raise new questions with respect to the underlying mechanism of ExsA regulation.
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{{STRUCTURE_3fd9| PDB=3fd9 | SCENE= }}
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Structural evidence suggests that antiactivator ExsD from Pseudomonas aeruginosa is a DNA binding protein.,Bernhards RC, Jing X, Vogelaar NJ, Robinson H, Schubot FD Protein Sci. 2009 Mar;18(3):503-13. PMID:19235906<ref>PMID:19235906</ref>
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===Crystal Structure of the transcriptional anti-activator ExsD from Pseudomonas aeruginosa===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19235906}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3fd9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_ucbpp-pa14 Pseudomonas aeruginosa ucbpp-pa14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FD9 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019235906</ref><references group="xtra"/>
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[[Category: Pseudomonas aeruginosa ucbpp-pa14]]
[[Category: Pseudomonas aeruginosa ucbpp-pa14]]
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[[Category: Schubot, F D.]]
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[[Category: Schubot, F D]]
[[Category: Anti-activator]]
[[Category: Anti-activator]]
[[Category: Pseudomona]]
[[Category: Pseudomona]]

Revision as of 06:58, 25 December 2014

Crystal Structure of the transcriptional anti-activator ExsD from Pseudomonas aeruginosa

3fd9, resolution 2.60Å

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