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3fdq

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[[Image:3fdq.png|left|200px]]
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==Recognition of AT-rich DNA binding sites by the MogR Repressor==
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<StructureSection load='3fdq' size='340' side='right' caption='[[3fdq]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3fdq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FDQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FDQ FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lmo0674, mogR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1639 Listeria monocytogenes])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fdq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fdq RCSB], [http://www.ebi.ac.uk/pdbsum/3fdq PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fd/3fdq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The MogR transcriptional repressor of the intracellular pathogen Listeria monocytogenes recognizes AT-rich binding sites in promoters of flagellar genes to downregulate flagellar gene expression during infection. We describe here the 1.8 A resolution crystal structure of MogR bound to the recognition sequence 5' ATTTTTTAAAAAAAT 3' present within the flaA promoter region. Our structure shows that MogR binds as a dimer. Each half-site is recognized in the major groove by a helix-turn-helix motif and in the minor groove by a loop from the symmetry-related molecule, resulting in a "crossover" binding mode. This oversampling through minor groove interactions is important for specificity. The MogR binding site has structural features of A-tract DNA and is bent by approximately 52 degrees away from the dimer. The structure explains how MogR achieves binding specificity in the AT-rich genome of L. monocytogenes and explains the evolutionary conservation of A-tract sequence elements within promoter regions of MogR-regulated flagellar genes.
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{{STRUCTURE_3fdq| PDB=3fdq | SCENE= }}
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Recognition of AT-rich DNA binding sites by the MogR repressor.,Shen A, Higgins DE, Panne D Structure. 2009 May 13;17(5):769-77. PMID:19446532<ref>PMID:19446532</ref>
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===Recognition of AT-rich DNA binding sites by the MogR Repressor===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19446532}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3fdq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FDQ OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019446532</ref><references group="xtra"/>
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[[Category: Listeria monocytogenes]]
[[Category: Listeria monocytogenes]]
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[[Category: Higgins, D E.]]
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[[Category: Higgins, D E]]
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[[Category: Panne, D.]]
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[[Category: Panne, D]]
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[[Category: Shen, A.]]
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[[Category: Shen, A]]
[[Category: Dna binding protein-dna complex]]
[[Category: Dna binding protein-dna complex]]
[[Category: Dna-binding]]
[[Category: Dna-binding]]

Revision as of 08:54, 26 November 2014

Recognition of AT-rich DNA binding sites by the MogR Repressor

3fdq, resolution 1.75Å

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