Subtilisin
From Proteopedia
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== 3D Structures of Subtilisin == | == 3D Structures of Subtilisin == | ||
| - | + | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | |
=== Native mature subtilisin === | === Native mature subtilisin === | ||
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[[2zwo]], [[2zwp]] – pro-TKSub | [[2zwo]], [[2zwp]] – pro-TKSub | ||
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=== Mature subtilisin complexed with its propeptide === | === Mature subtilisin complexed with its propeptide === | ||
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[[1spb]] – BaSub (mutant)<br /> | [[1spb]] – BaSub (mutant)<br /> | ||
[[3a3n]], [[3a3o]], [[3a3p]], [[2z56]], [[2z57]], [[2z58]], [[2z2y]], [[2z30]] – TkSub<br /> | [[3a3n]], [[3a3o]], [[3a3p]], [[2z56]], [[2z57]], [[2z58]], [[2z2y]], [[2z30]] – TkSub<br /> | ||
| + | [[3vv2]] – TkSub (mutant)<br /> | ||
[[1scj]] – BsSub | [[1scj]] – BsSub | ||
| - | |||
=== Subtilisin complexed with polypeptide inhibitor === | === Subtilisin complexed with polypeptide inhibitor === | ||
Revision as of 09:43, 22 October 2013
Subtilisin is a serine protease. It is widely used commercially in detergents. A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzyme. The images at the left and at the right correspond to one representative Subtilisin, i.e. the crystal structure of native mature subtilisin from Thermococcus kodakarensis (2z2x). See detalis in User:Tommie Hata/Introduction to Protein Engineering-Subtilisin.
3D Structures of Subtilisin
Updated on 22-October-2013
Native mature subtilisin
2z2x – TkSub - Thermococcus kodakarensis
1sca, 1scb, 1sbc, 3unx – BsSub - Bacillus subtilis (Subtilisin Carlsberg)
1pxq – BsSub - NMR
1gns, 1sup, 1st2, 1s01, 1sbt, 2sbt, 2st1 – BaSub - Bacillus amyloliquefaciens
1iav, 1gci, 1jea, 1st3 – BlSub - Bacillus lentus (Subtilisin Savinase)
1ea7, 3d43, 2ixt – spehricase – Sub from Bacillus sphaericus
2gko – Sub – psychrophilic Bacillus subtilis
Mutant mature subtilisin
3f49, 3co0, 2zrq, 3vhq – TkSub (mutant)
1gnv, 1dui, 1sue, 1a2q, 1aqn, 1sua, 1au9, 1ak9, 1yja, 1yjb, 1yjc, 1sbh, 1sbi, 1sub, 1suc, 1sud, 1s02 – BaSub (mutant)
1ndu, 1q5p, 1c9j, 1c9m, 1c9n – BlSub (mutant)
2xrm - BcSub residues 19-321 - Bacillus clausii
Unautoprocessed subtilisin (pro-Sub)
2z2z, 2e1p – pro-TkSub
2zwo, 2zwp – pro-TKSub (mutant)
2wv7 – pro-BcSub
2wwt, 2x8j - pro-BcSub (mutant)
Mutant unautoprocessed subtilisin
Mature subtilisin complexed with its propeptide
3cnq – BaSub
1spb – BaSub (mutant)
3a3n, 3a3o, 3a3p, 2z56, 2z57, 2z58, 2z2y, 2z30 – TkSub
3vv2 – TkSub (mutant)
1scj – BsSub
Subtilisin complexed with polypeptide inhibitor
1y1k, 1y33, 1y34, 1y3b, 1y3c, 1y3d, 1y3f, 1y48, 1y4a, 1y4d, 1tm3, 1tm4,
1tm1, 1lw6 – BaSub+ chymotripsin inhibitor 2
1tm5, 1tm7, 1tmg, 1to1, 1to2 - BaSub+ chymotrypsin inhibitor 2 (mutant)
3sic, 5sic, 2sic – BaSub+Streptomyces inhibitor SSI
1yu6 – BsSub+ovomucoid
1v5i – BaSubb+POIA1 peptide
1tk2, 1ndq – BlSub+gramicidin S
1r0r – BsSub+ovomucoid
1oyv – BsSub+tomato inhibitor II
1sbn, 1sib, 2sec, 1sni, 1sce – BsSub+Elgin C
2sni - BaSub+Elgin C2
1mee - BpSub+Elgin C – Bacillus pumilus
Subtilisin complexed with inhibitor
3bgo – BaSub+azide
1c3l – BsSub+Xe
1be8 – BsSub+cinnamoyl
1be6 - BsSub+cinnamoyl+acetonitrile
1bfk, 1scd – BsSub+acetonitrile
1bfu, 1af4 – BsSub+dioxane
1av7, 1avt, 3vsb, 1vsb – BsSub+boronic acid derivatives
1scn – BsSub+carbamate derivative
1bh6 – Sub DY+benzyloxycarbonyl-ala-pro-phe-chloromethyl ketone – Bacillus licheniformis
2wuv, 2wuw – BlSub fragment+acetonitrile
Selenosubtilisin
1ubn – BaSel-Sub
1sel – BsSel-Sub
Savinase
3bx1 – Sav – Bacillus lentus
