2a5x

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[[Image:2a5x.gif|left|200px]]<br /><applet load="2a5x" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2a5x.gif|left|200px]]
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caption="2a5x, resolution 2.49&Aring;" />
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'''Crystal Structure of a Cross-linked Actin Dimer'''<br />
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{{Structure
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|PDB= 2a5x |SIZE=350|CAPTION= <scene name='initialview01'>2a5x</scene>, resolution 2.49&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=LAR:LATRUNCULIN+A'>LAR</scene>, <scene name='pdbligand=NSB:N,N,N-TRIMETHYL-3-SULFOPROPAN-1-AMINIUM'>NSB</scene> and <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal Structure of a Cross-linked Actin Dimer'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2A5X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ANP:'>ANP</scene>, <scene name='pdbligand=LAR:'>LAR</scene>, <scene name='pdbligand=NSB:'>NSB</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5X OCA].
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2A5X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5X OCA].
==Reference==
==Reference==
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The crystal structure of a cross-linked actin dimer suggests a detailed molecular interface in F-actin., Kudryashov DS, Sawaya MR, Adisetiyo H, Norcross T, Hegyi G, Reisler E, Yeates TO, Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13105-10. Epub 2005 Sep 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16141336 16141336]
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The crystal structure of a cross-linked actin dimer suggests a detailed molecular interface in F-actin., Kudryashov DS, Sawaya MR, Adisetiyo H, Norcross T, Hegyi G, Reisler E, Yeates TO, Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13105-10. Epub 2005 Sep 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16141336 16141336]
[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: structural protein]]
[[Category: structural protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:45:47 2008''

Revision as of 13:45, 20 March 2008

Image:2a5x.gif


PDB ID 2a5x

Drag the structure with the mouse to rotate
, resolution 2.49Å
Ligands: , , , and
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of a Cross-linked Actin Dimer


Overview

The 2.5-A resolution crystal structure is reported for an actin dimer, composed of two protomers cross-linked along the longitudinal (or vertical) direction of the F-actin filament. The crystal structure provides an atomic resolution view of a molecular interface between actin protomers, which we argue represents a near-native interaction in the F-actin filament. The interaction involves subdomains 3 and 4 from distinct protomers. The atomic positions in the interface visualized differ by 5-10 A from those suggested by previous models of F-actin. Such differences fall within the range of uncertainties allowed by the fiber diffraction and electron microscopy methods on which previous models have been based. In the crystal, the translational arrangement of protomers lacks the slow twist found in native filaments. A plausible model of F-actin can be constructed by reintroducing the known filament twist, without disturbing significantly the interface observed in the actin dimer crystal.

About this Structure

2A5X is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

Reference

The crystal structure of a cross-linked actin dimer suggests a detailed molecular interface in F-actin., Kudryashov DS, Sawaya MR, Adisetiyo H, Norcross T, Hegyi G, Reisler E, Yeates TO, Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13105-10. Epub 2005 Sep 1. PMID:16141336

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