2a8m

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2a8m.gif|left|200px]]<br /><applet load="2a8m" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:2a8m.gif|left|200px]]
-
caption="2a8m, resolution 2.60&Aring;" />
+
 
-
'''Crystal Structure of Human Taspase1 (T234S mutant)'''<br />
+
{{Structure
 +
|PDB= 2a8m |SIZE=350|CAPTION= <scene name='initialview01'>2a8m</scene>, resolution 2.60&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
 +
|ACTIVITY=
 +
|GENE= C20orf13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
 +
}}
 +
 
 +
'''Crystal Structure of Human Taspase1 (T234S mutant)'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
2A8M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA].
+
2A8M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8M OCA].
==Reference==
==Reference==
-
Crystal structure of human Taspase1, a crucial protease regulating the function of MLL., Khan JA, Dunn BM, Tong L, Structure. 2005 Oct;13(10):1443-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16216576 16216576]
+
Crystal structure of human Taspase1, a crucial protease regulating the function of MLL., Khan JA, Dunn BM, Tong L, Structure. 2005 Oct;13(10):1443-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16216576 16216576]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 22: Line 31:
[[Category: taspase]]
[[Category: taspase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:24:51 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:46:44 2008''

Revision as of 13:46, 20 March 2008

Image:2a8m.gif


PDB ID 2a8m

Drag the structure with the mouse to rotate
, resolution 2.60Å
Ligands:
Gene: C20orf13 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Human Taspase1 (T234S mutant)


Overview

Taspase1 catalyzes the proteolytic processing of the mixed lineage leukemia (MLL) nuclear protein, which is required for maintaining Hox gene expression patterns. Chromosomal translocations of the MLL gene are associated with leukemia in infants. Taspase1, a threonine aspartase, is a member of the type 2 asparaginase family, but is the only protease in this family. We report here the crystal structures of human activated Taspase1 and its proenzyme, as well as the characterization of the effects of mutations in the active site region using a newly developed fluorogenic assay. The structure of Taspase1 has significant differences from other asparaginases, especially near the active site. Mutation of the catalytic nucleophile, Thr234, abolishes autocatalytic processing in cis but does not completely block proteolysis in trans. The structure unexpectedly showed the binding of a chloride ion in the active site, and our kinetic studies confirm that chlorides ions are inhibitors of this enzyme at physiologically relevant concentrations.

About this Structure

2A8M is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human Taspase1, a crucial protease regulating the function of MLL., Khan JA, Dunn BM, Tong L, Structure. 2005 Oct;13(10):1443-52. PMID:16216576

Page seeded by OCA on Thu Mar 20 15:46:44 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2a8m"
Personal tools