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3fqq

From Proteopedia

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[[Image:3fqq.png|left|200px]]
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==Crystal structure of a novel dimeric form of HCV NS5A domain I protein==
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<StructureSection load='3fqq' size='340' side='right' caption='[[3fqq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3fqq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FQQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FQQ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0BD:3-METHYL-1-(2-METHYLPROPYL)BUTYL+4-O-BETA-L-GULOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE'>0BD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zh1|1zh1]], [[2fqm|2fqm]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Nonstructural protein NS5A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10239 Viruses])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fqq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fqq RCSB], [http://www.ebi.ac.uk/pdbsum/3fqq PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fq/3fqq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A new protein expression vector design utilizing an N-terminal six-histidine tag and tobacco etch virus protease cleavage site upstream of the hepatitis C virus NS5A sequence has resulted in a more straightforward purification method and improved yields of purified NS5A domain I protein. High-resolution diffracting crystals of NS5A domain I (amino acids 33 to 202) [NS5A(33-202)] were obtained by using detergent additive crystallization screens, leading to the structure of a homodimer which is organized differently from that published previously (T. L. Tellinghuisen, J. Marcotrigiano, and C. M. Rice, Nature 435:374-379, 2005) yet is consistent with a membrane association model for NS5A. The monomer-monomer interface of NS5A(33-202) features an extensive buried surface area involving the most-highly conserved face of each monomer. The two alternate structural forms of domain I now available may be indicative of the multiple roles emerging for NS5A in viral RNA replication and viral particle assembly.
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{{STRUCTURE_3fqq| PDB=3fqq | SCENE= }}
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Crystal structure of a novel dimeric form of NS5A domain I protein from hepatitis C virus.,Love RA, Brodsky O, Hickey MJ, Wells PA, Cronin CN J Virol. 2009 May;83(9):4395-403. Epub 2009 Feb 25. PMID:19244328<ref>PMID:19244328</ref>
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===Crystal structure of a novel dimeric form of HCV NS5A domain I protein===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19244328}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3fqq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FQQ OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019244328</ref><references group="xtra"/>
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[[Category: Viruses]]
[[Category: Viruses]]
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[[Category: Love, R A.]]
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[[Category: Love, R A]]
[[Category: Domain i]]
[[Category: Domain i]]
[[Category: Hcv]]
[[Category: Hcv]]

Revision as of 10:14, 3 December 2014

Crystal structure of a novel dimeric form of HCV NS5A domain I protein

3fqq, resolution 2.20Å

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