This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3fpx
From Proteopedia
(Difference between revisions)
m (Protected "3fpx" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | [[ | + | ==Native fungus laccase from Trametes hirsuta== |
| + | <StructureSection load='3fpx' size='340' side='right' caption='[[3fpx]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3fpx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trametes_hirsuta Trametes hirsuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FPX FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fpx RCSB], [http://www.ebi.ac.uk/pdbsum/3fpx PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/3fpx_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Laccase from the fungus Coriolus hirsutus has been purified. Crystals of the enzyme suitable for X-ray structure analysis have been obtained under optimized crystallization conditions using polyethylene glycol as a precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.65, b = 74.01, c = 124.83 A, and contain 40% solvent and a single molecule of laccase in the asymmetric unit. X-ray data were collected to 1.85 A at the copper edge and the four copper sites have been located from the anomalous signal. The obtained SAD phases with subsequent density modification produced a promising initial electron-density map. | ||
| - | + | Crystallization and preliminary X-ray analysis of a four-copper laccase from Coriolus hirsutus.,Pegasova TV, Zwart P, Koroleva OV, Stepanova EV, Rebrikov DV, Lamzin VS Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1459-61. Epub 2003, Jul 23. PMID:12876350<ref>PMID:12876350</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Laccase]] | [[Category: Laccase]] | ||
[[Category: Trametes hirsuta]] | [[Category: Trametes hirsuta]] | ||
| - | [[Category: Cherkashin, E A | + | [[Category: Cherkashin, E A]] |
| - | [[Category: Fedorova, T V | + | [[Category: Fedorova, T V]] |
| - | [[Category: Koroleva, O V | + | [[Category: Koroleva, O V]] |
| - | [[Category: Kurzeev, S A | + | [[Category: Kurzeev, S A]] |
| - | [[Category: Lamzin, V S | + | [[Category: Lamzin, V S]] |
| - | [[Category: Polyakov, K M | + | [[Category: Polyakov, K M]] |
| - | [[Category: Stepanova, E V | + | [[Category: Stepanova, E V]] |
| - | [[Category: Strokopytov, B V | + | [[Category: Strokopytov, B V]] |
[[Category: 4-copper protein]] | [[Category: 4-copper protein]] | ||
[[Category: Beta sheet]] | [[Category: Beta sheet]] | ||
[[Category: Metal-binding]] | [[Category: Metal-binding]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
Revision as of 10:23, 3 December 2014
Native fungus laccase from Trametes hirsuta
| |||||||||||
