2abw

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[[Image:2abw.gif|left|200px]]<br /><applet load="2abw" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2abw.gif|left|200px]]
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caption="2abw, resolution 1.620&Aring;" />
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'''Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)'''<br />
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{{Structure
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|PDB= 2abw |SIZE=350|CAPTION= <scene name='initialview01'>2abw</scene>, resolution 1.620&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PG4:TETRAETHYLENE GLYCOL'>PG4</scene>
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|ACTIVITY=
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|GENE= Pdx2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum])
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}}
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'''Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2ABW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum] with <scene name='pdbligand=PG4:'>PG4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABW OCA].
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2ABW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABW OCA].
==Reference==
==Reference==
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Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights., Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Muller S, Macheroux P, Tews I, Kappes B, J Biol Chem. 2006 Feb 10;281(6):3633-41. Epub 2005 Dec 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16339145 16339145]
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Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights., Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Muller S, Macheroux P, Tews I, Kappes B, J Biol Chem. 2006 Feb 10;281(6):3633-41. Epub 2005 Dec 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16339145 16339145]
[[Category: Plasmodium falciparum]]
[[Category: Plasmodium falciparum]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: vitamin b6]]
[[Category: vitamin b6]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:25:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:47:49 2008''

Revision as of 13:47, 20 March 2008


PDB ID 2abw

Drag the structure with the mouse to rotate
, resolution 1.620Å
Ligands:
Gene: Pdx2 (Plasmodium falciparum)
Coordinates: save as pdb, mmCIF, xml



Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)


Overview

Vitamin B6 is one of nature's most versatile cofactors. Most organisms synthesize vitamin B6 via a recently discovered pathway employing the proteins Pdx1 and Pdx2. Here we present an in-depth characterization of the respective orthologs from the malaria parasite, Plasmodium falciparum. Expression profiling of Pdx1 and -2 shows that blood-stage parasites indeed possess a functional vitamin B6 de novo biosynthesis. Recombinant Pdx1 and Pdx2 form a complex that functions as a glutamine amidotransferase with Pdx2 as the glutaminase and Pdx1 as pyridoxal-5 '-phosphate synthase domain. Complex formation is required for catalytic activity of either domain. Pdx1 forms a chimeric bi-enzyme with the bacterial YaaE, a Pdx2 ortholog, both in vivo and in vitro, although this chimera does not attain full catalytic activity, emphasizing that species-specific structural features govern the interaction between the protein partners of the PLP synthase complexes in different organisms. To gain insight into the activation mechanism of the parasite bi-enzyme complex, the three-dimensional structure of Pdx2 was determined at 1.62 A. The obstruction of the oxyanion hole indicates that Pdx2 is in a resting state and that activation occurs upon Pdx1-Pdx2 complex formation.

About this Structure

2ABW is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.

Reference

Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights., Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Muller S, Macheroux P, Tews I, Kappes B, J Biol Chem. 2006 Feb 10;281(6):3633-41. Epub 2005 Dec 8. PMID:16339145

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