2abs
From Proteopedia
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| - | [[Image:2abs.gif|left|200px]] | + | [[Image:2abs.gif|left|200px]] |
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| - | '''Crystal structure of T. gondii adenosine kinase complexed with AMP-PCP''' | + | {{Structure |
| + | |PDB= 2abs |SIZE=350|CAPTION= <scene name='initialview01'>2abs</scene>, resolution 1.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER'>ACP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] | ||
| + | |GENE= AK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 Toxoplasma gondii]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of T. gondii adenosine kinase complexed with AMP-PCP''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ABS is a [ | + | 2ABS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABS OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of Toxoplasma gondii adenosine kinase in complex with an ATP analog at 1.1 angstroms resolution., Zhang Y, El Kouni MH, Ealick SE, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):140-5. Epub 2006, Jan 18. PMID:[http:// | + | Structure of Toxoplasma gondii adenosine kinase in complex with an ATP analog at 1.1 angstroms resolution., Zhang Y, El Kouni MH, Ealick SE, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):140-5. Epub 2006, Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16421444 16421444] |
[[Category: Adenosine kinase]] | [[Category: Adenosine kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ribokinase fold; alpha/beta; intermediate conformation]] | [[Category: ribokinase fold; alpha/beta; intermediate conformation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:47:50 2008'' |
Revision as of 13:47, 20 March 2008
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| , resolution 1.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | AK (Toxoplasma gondii) | ||||||
| Activity: | Adenosine kinase, with EC number 2.7.1.20 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of T. gondii adenosine kinase complexed with AMP-PCP
Overview
The obligate intracellular parasite Toxoplasma gondii is incapable of synthesizing purine nucleotides de novo and relies completely on purines salvaged from the host cells. Adenosine is the preferred precursor and is phosphorylated by adenosine kinase (AK), the most active enzyme in adenosine metabolism in T. gondii. AK thus represents a potential chemotherapeutic target for the treatment of T. gondii infections. The previously solved structures of unliganded AK and AK in complex with adenosine (or 7-iodotubercidin) and an ATP analog revealed a novel catalytic mechanism. A domain closure triggered by a GG switch upon adenosine binding sequesters the adenosine and gamma-phosphate of ATP from the solvent. The formation of the anion hole induced by the ATP binding completes the structural requirements for catalysis. In the current study, the structure of a binary complex of AK and the non-hydrolysable ATP analog AMP-PCP was determined to 1.1 angstroms resolution. The overall structure is similar to the apoenzyme, with an open conformation. AMP-PCP is bound in two relaxed conformations and without anchoring by Arg136. The induced anion hole is the same as that in the ternary complex AK-adenosine-AMP-PCP. This structure provides direct evidence that ATP binding at millimolar concentrations does not require adenosine binding as a prerequisite.
About this Structure
2ABS is a Single protein structure of sequence from Toxoplasma gondii. Full crystallographic information is available from OCA.
Reference
Structure of Toxoplasma gondii adenosine kinase in complex with an ATP analog at 1.1 angstroms resolution., Zhang Y, El Kouni MH, Ealick SE, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):140-5. Epub 2006, Jan 18. PMID:16421444
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