2acj
From Proteopedia
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| - | [[Image:2acj.gif|left|200px]] | + | [[Image:2acj.gif|left|200px]] |
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| - | '''Crystal structure of the B/Z junction containing DNA bound to Z-DNA binding proteins''' | + | {{Structure |
| + | |PDB= 2acj |SIZE=350|CAPTION= <scene name='initialview01'>2acj</scene>, resolution 2.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= ADAR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the B/Z junction containing DNA bound to Z-DNA binding proteins''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ACJ is a [ | + | 2ACJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ACJ OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a junction between B-DNA and Z-DNA reveals two extruded bases., Ha SC, Lowenhaupt K, Rich A, Kim YG, Kim KK, Nature. 2005 Oct 20;437(7062):1183-6. PMID:[http:// | + | Crystal structure of a junction between B-DNA and Z-DNA reveals two extruded bases., Ha SC, Lowenhaupt K, Rich A, Kim YG, Kim KK, Nature. 2005 Oct 20;437(7062):1183-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16237447 16237447] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:48:00 2008'' |
Revision as of 13:48, 20 March 2008
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| , resolution 2.60Å | |||||||
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| Gene: | ADAR1 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the B/Z junction containing DNA bound to Z-DNA binding proteins
Contents |
Overview
Left-handed Z-DNA is a higher-energy form of the double helix, stabilized by negative supercoiling generated by transcription or unwrapping nucleosomes. Regions near the transcription start site frequently contain sequence motifs favourable for forming Z-DNA, and formation of Z-DNA near the promoter region stimulates transcription. Z-DNA is also stabilized by specific protein binding; several proteins have been identified with low nanomolar binding constants. Z-DNA occurs in a dynamic state, forming as a result of physiological processes then relaxing to the right-handed B-DNA. Each time a DNA segment turns into Z-DNA, two B-Z junctions form. These have been examined extensively, but their structure was unknown. Here we describe the structure of a B-Z junction as revealed by X-ray crystallography at 2.6 A resolution. A 15-base-pair segment of DNA is stabilized at one end in the Z conformation by Z-DNA binding proteins, while the other end remains B-DNA. Continuous stacking of bases between B-DNA and Z-DNA segments is found, with the breaking of one base pair at the junction and extrusion of the bases on each side (Fig. 1). These extruded bases may be sites for DNA modification.
Disease
Known diseases associated with this structure: Dyschromatosis symmetrica hereditaria OMIM:[601059]
About this Structure
2ACJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a junction between B-DNA and Z-DNA reveals two extruded bases., Ha SC, Lowenhaupt K, Rich A, Kim YG, Kim KK, Nature. 2005 Oct 20;437(7062):1183-6. PMID:16237447
Page seeded by OCA on Thu Mar 20 15:48:00 2008
