This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4fgn
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [[ | + | ==Crystal structure of the SV40 large T-antigen origin bining domain bound to Site I DNA== |
| + | <StructureSection load='4fgn' size='340' side='right' caption='[[4fgn]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4fgn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FGN FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2fuf|2fuf]], [[2ntc|2ntc]], [[2itl|2itl]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fgn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fgn RCSB], [http://www.ebi.ac.uk/pdbsum/4fgn PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Polyomavirus origins of replication contain multiple G(A/G)GGC sequences; the high affinity binding element for the viral initiator T-antigen (T-ag). The Site I regulatory region of Simian Virus 40, involved in the repression of transcription and the enhancement of DNA replication initiation, contains two GAGGC sequences arranged head-to-tail and separated by a 7 bp AT-rich sequence. We have solved a 3.2 A co-structure of the SV40 origin-binding domain (OBD) bound to Site I. We have also established that T-ag assembly on Site I is limited to the formation of a single hexamer. These observations have enabled an analyses of the role(s) of the OBDs bound to the Site I pentanucleotides in hexamer formation. Of interest, they reveal a correlation between the OBDs bound to Site I and a pair of OBD subunits in the previously described hexameric spiral structure. Based on these findings, we propose that spiral assembly is promoted by pentanucleotide pairs arranged in a head to tail manner. Finally, the possibility that "spiral-assembly " by OBD subunits may account for the heterogeneous distribution of pentanucleotides found in the origins of replication of polyomaviruses is discussed. | ||
| - | + | ANALYSIS OF THE CO-STRUCTURE OF THE SV40 T-ANTIGEN ORIGIN BINDING DOMAIN WITH SITE I REVEALS A CORRELATION BETWEEN GAGGC SPACING AND SPIRAL ASSEMBLY.,Meinke G, Phelan PJ, Harrison C, Bullock PA J Virol. 2012 Dec 26. PMID:23269808<ref>PMID:23269808</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | + | *[[Large T Antigen|Large T Antigen]] | |
| - | == | + | == References == |
| - | [[ | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Simian virus 40]] | [[Category: Simian virus 40]] | ||
| - | [[Category: Bohm, A | + | [[Category: Bohm, A]] |
| - | [[Category: Bullock, P A | + | [[Category: Bullock, P A]] |
| - | [[Category: Meinke, G | + | [[Category: Meinke, G]] |
[[Category: Dna binding protein-dna complex]] | [[Category: Dna binding protein-dna complex]] | ||
[[Category: Origin binding domain]] | [[Category: Origin binding domain]] | ||
Revision as of 17:41, 9 December 2014
Crystal structure of the SV40 large T-antigen origin bining domain bound to Site I DNA
| |||||||||||
