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2aga

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[[Image:2aga.gif|left|200px]]<br /><applet load="2aga" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2aga.gif|left|200px]]
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caption="2aga" />
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'''De-ubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain'''<br />
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{{Structure
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|PDB= 2aga |SIZE=350|CAPTION= <scene name='initialview01'>2aga</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= ATXN3, ATX3, MJD, MJD1, SCA3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''De-ubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2AGA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AGA OCA].
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2AGA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AGA OCA].
==Reference==
==Reference==
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Deubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain., Mao Y, Senic-Matuglia F, Di Fiore PP, Polo S, Hodsdon ME, De Camilli P, Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12700-5. Epub 2005 Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16118278 16118278]
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Deubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain., Mao Y, Senic-Matuglia F, Di Fiore PP, Polo S, Hodsdon ME, De Camilli P, Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12700-5. Epub 2005 Aug 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16118278 16118278]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: vcp/p97]]
[[Category: vcp/p97]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:27:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:49:23 2008''

Revision as of 13:49, 20 March 2008


PDB ID 2aga

Drag the structure with the mouse to rotate
Gene: ATXN3, ATX3, MJD, MJD1, SCA3 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



De-ubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain


Contents

Overview

Spinocerebellar ataxia type 3 is a human neurodegenerative disease resulting from polyglutamine tract expansion. The affected protein, ataxin-3, which contains an N-terminal Josephin domain followed by tandem ubiquitin (Ub)-interacting motifs (UIMs) and a polyglutamine stretch, has been implicated in the function of the Ub proteasome system. NMR-based structural analysis has now revealed that the Josephin domain binds Ub and has a papain-like fold that is reminiscent of that of other deubiquitinases, despite primary sequence divergence but consistent with its deubiqutinating activity. Mutation of the catalytic Cys enhances the stability of a complex between ataxin-3 and polyubiquitinated proteins. This effect depends on the integrity of the UIM region, suggesting that the UIMs are bound to the substrate polyubiquitin during catalysis. We propose that ataxin-3 functions as a polyubiquitin chain-editing enzyme.

Disease

Known diseases associated with this structure: Machado-Joseph disease OMIM:[607047]

About this Structure

2AGA is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Deubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain., Mao Y, Senic-Matuglia F, Di Fiore PP, Polo S, Hodsdon ME, De Camilli P, Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12700-5. Epub 2005 Aug 23. PMID:16118278

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