2ahc
From Proteopedia
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| - | [[Image:2ahc.gif|left|200px]] | + | [[Image:2ahc.gif|left|200px]] |
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| - | '''Chorismate lyase with inhibitor Vanilate''' | + | {{Structure |
| + | |PDB= 2ahc |SIZE=350|CAPTION= <scene name='initialview01'>2ahc</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=VNL:4-HYDROXY-3-METHOXYBENZOATE'>VNL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= ubiC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Chorismate lyase with inhibitor Vanilate''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2AHC is a [ | + | 2AHC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHC OCA]. |
==Reference== | ==Reference== | ||
| - | Structural analysis of ligand binding and catalysis in chorismate lyase., Smith N, Roitberg AE, Rivera E, Howard A, Holden MJ, Mayhew M, Kaistha S, Gallagher DT, Arch Biochem Biophys. 2006 Jan 1;445(1):72-80. Epub 2005 Nov 22. PMID:[http:// | + | Structural analysis of ligand binding and catalysis in chorismate lyase., Smith N, Roitberg AE, Rivera E, Howard A, Holden MJ, Mayhew M, Kaistha S, Gallagher DT, Arch Biochem Biophys. 2006 Jan 1;445(1):72-80. Epub 2005 Nov 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16343413 16343413] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: unique fold]] | [[Category: unique fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:49:41 2008'' |
Revision as of 13:49, 20 March 2008
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| , resolution 2.40Å | |||||||
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| Ligands: | |||||||
| Gene: | ubiC (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Chorismate lyase with inhibitor Vanilate
Overview
Chorismate lyase (CL) removes the pyruvyl group from chorismate to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway. We previously reported the crystal structure at 1.4A resolution of the Escherichia coli CL with bound 4HB product, showing that the product is bound in an internal cavity behind two flaps. To provide a more complete basis for understanding CL's unusual ligand-binding properties and mechanism of action, we now report four crystal structures of CL mutants and inhibitor complexes, together with binding and activity measurements and molecular dynamics simulations. First, an ultrahigh resolution (1.0A) crystal structure of the CL*product complex reveals details of a substrate-sized internal cavity, also behind the flaps, near the product site. Second, a 2.4A structure of CL complexed with the inhibitor vanillate shows the flaps partly opened relative to their product-bound positions. Third, a 2.0A structure of the G90A mutant with bound product reveals the basis for tighter product binding and kinetic effects of this active site mutation. Fourth, the combination of the G90A mutation with the vanillate inhibitor produces a 1.9A structure containing two inhibitor molecules, one in the product site and the other in the adjacent cavity. The two sites are connected by a short tunnel that is partly open at each end, suggesting that CL may operate via a 2-site or tunnel mechanism.
About this Structure
2AHC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural analysis of ligand binding and catalysis in chorismate lyase., Smith N, Roitberg AE, Rivera E, Howard A, Holden MJ, Mayhew M, Kaistha S, Gallagher DT, Arch Biochem Biophys. 2006 Jan 1;445(1):72-80. Epub 2005 Nov 22. PMID:16343413
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