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2ahj
From Proteopedia
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| - | [[Image:2ahj.gif|left|200px]] | + | [[Image:2ahj.gif|left|200px]] |
| - | + | ||
| - | '''NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE''' | + | {{Structure |
| + | |PDB= 2ahj |SIZE=350|CAPTION= <scene name='initialview01'>2ahj</scene>, resolution 1.7Å | ||
| + | |SITE= <scene name='pdbsite=1:In+Chain+C'>1</scene>, <scene name='pdbsite=CTA:Non-Heme+Fe+Center+And+Catalytic+Site+Site_description+I+...'>CTA</scene> and <scene name='pdbsite=CTB:In+Chain+A+Site_identifier+Ctb+Site_description+Non-Heme+...'>CTB</scene> | ||
| + | |LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NO:NITROGEN+OXIDE'>NO</scene> and <scene name='pdbligand=DIO:1,4-DIETHYLENE DIOXIDE'>DIO</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2AHJ is a [ | + | 2AHJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHJ OCA]. |
==Reference== | ==Reference== | ||
| - | Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms., Nagashima S, Nakasako M, Dohmae N, Tsujimura M, Takio K, Odaka M, Yohda M, Kamiya N, Endo I, Nat Struct Biol. 1998 May;5(5):347-51. PMID:[http:// | + | Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms., Nagashima S, Nakasako M, Dohmae N, Tsujimura M, Takio K, Odaka M, Yohda M, Kamiya N, Endo I, Nat Struct Biol. 1998 May;5(5):347-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9586994 9586994] |
[[Category: Nitrile hydratase]] | [[Category: Nitrile hydratase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: non-heme iron center]] | [[Category: non-heme iron center]] | ||
[[Category: photoreactive enzyme]] | [[Category: photoreactive enzyme]] | ||
| - | [[Category: post-translational modification of cysteine | + | [[Category: post-translational modification of cysteine residue]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:49:44 2008'' |
Revision as of 13:49, 20 March 2008
| |||||||
| , resolution 1.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , and | ||||||
| Ligands: | , , , and | ||||||
| Activity: | Nitrile hydratase, with EC number 4.2.1.84 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE
Overview
The iron-containing nitrile hydratase (NHase) is a photoreactive enzyme that is inactivated in the dark because of persistent association with NO and activated by photo-dissociation of NO. The crystal structure at 1.7 A resolution and mass spectrometry revealed the structure of the non-heme iron catalytic center in the nitrosylated state. Two Cys residues coordinated to the iron were post-translationally modified to Cys-sulfenic and -sulfinic acids. Together with another oxygen atom of the Ser ligand, these modifications induced a claw setting of oxygen atoms capturing an NO molecule. This unprecedented structure is likely to enable the photo-regulation of NHase and will provide an excellent model for designing photo-controllable chelate complexes and, ultimately, proteins.
About this Structure
2AHJ is a Protein complex structure of sequences from Rhodococcus erythropolis. Full crystallographic information is available from OCA.
Reference
Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms., Nagashima S, Nakasako M, Dohmae N, Tsujimura M, Takio K, Odaka M, Yohda M, Kamiya N, Endo I, Nat Struct Biol. 1998 May;5(5):347-51. PMID:9586994
Page seeded by OCA on Thu Mar 20 15:49:44 2008
Categories: Nitrile hydratase | Protein complex | Rhodococcus erythropolis | Dohmae, N. | Endo, I. | Kamiya, N. | Nagashima, S. | Nakasako, M. | Odaka, M. | Takio, K. | Tsujimura, M. | Yohda, M. | DIO | FE | NO | SO4 | ZN | Hydratase | Lyase | Nitric oxide binding enzyme | Non-heme iron center | Photoreactive enzyme | Post-translational modification of cysteine residue
