This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2aiq
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2aiq.gif|left|200px]] | + | [[Image:2aiq.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of benzamidine-inhibited protein C activator from the venom of copperhead snake Agkistrodon contortrix contortrix''' | + | {{Structure |
| + | |PDB= 2aiq |SIZE=350|CAPTION= <scene name='initialview01'>2aiq</scene>, resolution 1.54Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Venombin_A Venombin A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.74 3.4.21.74] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of benzamidine-inhibited protein C activator from the venom of copperhead snake Agkistrodon contortrix contortrix''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2AIQ is a [ | + | 2AIQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Agkistrodon_contortrix_contortrix Agkistrodon contortrix contortrix]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AIQ OCA]. |
==Reference== | ==Reference== | ||
| - | Thrombomodulin-independent activation of protein C and specificity of hemostatically active snake venom serine proteinases: crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator., Murakami MT, Arni RK, J Biol Chem. 2005 Nov 25;280(47):39309-15. Epub 2005 Sep 14. PMID:[http:// | + | Thrombomodulin-independent activation of protein C and specificity of hemostatically active snake venom serine proteinases: crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator., Murakami MT, Arni RK, J Biol Chem. 2005 Nov 25;280(47):39309-15. Epub 2005 Sep 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16162508 16162508] |
[[Category: Agkistrodon contortrix contortrix]] | [[Category: Agkistrodon contortrix contortrix]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 26: | Line 35: | ||
[[Category: snake venom serine proteinase]] | [[Category: snake venom serine proteinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:50:11 2008'' |
Revision as of 13:50, 20 March 2008
| |||||||
| , resolution 1.54Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , and | ||||||
| Activity: | Venombin A, with EC number 3.4.21.74 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of benzamidine-inhibited protein C activator from the venom of copperhead snake Agkistrodon contortrix contortrix
Overview
Protein C activation initiated by the thrombin-thrombomodulin complex forms the major physiological anticoagulant pathway. Agkistrodon contortrix contortrix protein C activator, a glycosylated single-chain serine proteinase, activates protein C without relying on thrombomodulin. The crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator determined at 1.65 and 1.54 A resolutions, respectively, indicate the pivotal roles played by the positively charged belt and the strategic positioning of the three carbohydrate moieties surrounding the catalytic site in protein C recognition, binding, and activation. Structural changes in the benzamidine-inhibited enzyme suggest a probable function in allosteric regulation for the anion-binding site located in the C-terminal extension, which is fully conserved in snake venom serine proteinases, that preferentially binds Cl(1-) instead of SO(4)(2-).
About this Structure
2AIQ is a Single protein structure of sequence from Agkistrodon contortrix contortrix. Full crystallographic information is available from OCA.
Reference
Thrombomodulin-independent activation of protein C and specificity of hemostatically active snake venom serine proteinases: crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator., Murakami MT, Arni RK, J Biol Chem. 2005 Nov 25;280(47):39309-15. Epub 2005 Sep 14. PMID:16162508
Page seeded by OCA on Thu Mar 20 15:50:11 2008
Categories: Agkistrodon contortrix contortrix | Single protein | Venombin A | Arni, R K. | Murakami, M T. | ACT | BEN | CL | GOL | NAG | NDG | SO4 | Protein c activator | Snake venom serine proteinase
