2aiq
From Proteopedia
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| - | [[Image:2aiq.gif|left|200px]] | + | [[Image:2aiq.gif|left|200px]] |
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| - | '''Crystal structure of benzamidine-inhibited protein C activator from the venom of copperhead snake Agkistrodon contortrix contortrix''' | + | {{Structure |
| + | |PDB= 2aiq |SIZE=350|CAPTION= <scene name='initialview01'>2aiq</scene>, resolution 1.54Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Venombin_A Venombin A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.74 3.4.21.74] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of benzamidine-inhibited protein C activator from the venom of copperhead snake Agkistrodon contortrix contortrix''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2AIQ is a [ | + | 2AIQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Agkistrodon_contortrix_contortrix Agkistrodon contortrix contortrix]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AIQ OCA]. |
==Reference== | ==Reference== | ||
| - | Thrombomodulin-independent activation of protein C and specificity of hemostatically active snake venom serine proteinases: crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator., Murakami MT, Arni RK, J Biol Chem. 2005 Nov 25;280(47):39309-15. Epub 2005 Sep 14. PMID:[http:// | + | Thrombomodulin-independent activation of protein C and specificity of hemostatically active snake venom serine proteinases: crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator., Murakami MT, Arni RK, J Biol Chem. 2005 Nov 25;280(47):39309-15. Epub 2005 Sep 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16162508 16162508] |
[[Category: Agkistrodon contortrix contortrix]] | [[Category: Agkistrodon contortrix contortrix]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: snake venom serine proteinase]] | [[Category: snake venom serine proteinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:50:11 2008'' |
Revision as of 13:50, 20 March 2008
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| , resolution 1.54Å | |||||||
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| Ligands: | , , , , , and | ||||||
| Activity: | Venombin A, with EC number 3.4.21.74 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of benzamidine-inhibited protein C activator from the venom of copperhead snake Agkistrodon contortrix contortrix
Overview
Protein C activation initiated by the thrombin-thrombomodulin complex forms the major physiological anticoagulant pathway. Agkistrodon contortrix contortrix protein C activator, a glycosylated single-chain serine proteinase, activates protein C without relying on thrombomodulin. The crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator determined at 1.65 and 1.54 A resolutions, respectively, indicate the pivotal roles played by the positively charged belt and the strategic positioning of the three carbohydrate moieties surrounding the catalytic site in protein C recognition, binding, and activation. Structural changes in the benzamidine-inhibited enzyme suggest a probable function in allosteric regulation for the anion-binding site located in the C-terminal extension, which is fully conserved in snake venom serine proteinases, that preferentially binds Cl(1-) instead of SO(4)(2-).
About this Structure
2AIQ is a Single protein structure of sequence from Agkistrodon contortrix contortrix. Full crystallographic information is available from OCA.
Reference
Thrombomodulin-independent activation of protein C and specificity of hemostatically active snake venom serine proteinases: crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator., Murakami MT, Arni RK, J Biol Chem. 2005 Nov 25;280(47):39309-15. Epub 2005 Sep 14. PMID:16162508
Page seeded by OCA on Thu Mar 20 15:50:11 2008
Categories: Agkistrodon contortrix contortrix | Single protein | Venombin A | Arni, R K. | Murakami, M T. | ACT | BEN | CL | GOL | NAG | NDG | SO4 | Protein c activator | Snake venom serine proteinase
