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3gwh
From Proteopedia
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| - | [[ | + | ==Crystallographic Ab Initio protein solution far below atomic resolution== |
| + | <StructureSection load='3gwh' size='340' side='right' caption='[[3gwh]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3gwh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GWH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GWH FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BSU13880, glcT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gwh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3gwh RCSB], [http://www.ebi.ac.uk/pdbsum/3gwh PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gw/3gwh_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Ab initio macromolecular phasing has been so far limited to small proteins diffracting at atomic resolution (beyond 1.2 A) unless heavy atoms are present. We describe a general ab initio phasing method for 2 A data, based on combination of localizing model fragments such as small a-helices with Phaser and density modification with SHELXE. We implemented this approach in the program Arcimboldo to solve a 222-amino-acid structure at 1.95 A. | ||
| - | + | Crystallographic ab initio protein structure solution below atomic resolution.,Rodriguez DD, Grosse C, Himmel S, Gonzalez C, de Ilarduya IM, Becker S, Sheldrick GM, Uson I Nat Methods. 2009 Sep;6(9):651-3. Epub 2009 Aug 16. PMID:19684596<ref>PMID:19684596</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
| - | [[Category: Becker, S | + | [[Category: Becker, S]] |
| - | [[Category: Gonzalez, C | + | [[Category: Gonzalez, C]] |
| - | [[Category: Grosse, C | + | [[Category: Grosse, C]] |
| - | [[Category: Himmel, S | + | [[Category: Himmel, S]] |
| - | [[Category: Rodriguez, D D | + | [[Category: Rodriguez, D D]] |
| - | [[Category: Sheldrick, G M | + | [[Category: Sheldrick, G M]] |
| - | [[Category: Uson, I | + | [[Category: Uson, I]] |
[[Category: Ab initio]] | [[Category: Ab initio]] | ||
[[Category: Arcimboldo]] | [[Category: Arcimboldo]] | ||
Revision as of 09:06, 8 December 2014
Crystallographic Ab Initio protein solution far below atomic resolution
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