4g9s
From Proteopedia
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| - | [[ | + | ==Crystal structure of Escherichia coli PliG in complex with Atlantic salmon g-type lysozyme== |
| + | <StructureSection load='4g9s' size='340' side='right' caption='[[4g9s]], [[Resolution|resolution]] 0.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4g9s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Salmo_salar Salmo salar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G9S FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lysG, LYG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8030 Salmo salar]), pliG, ycgK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g9s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g9s RCSB], [http://www.ebi.ac.uk/pdbsum/4g9s PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/PLIG_ECOLI PLIG_ECOLI]] Inhibits activity of g-type lysozyme, which confers increased lysozyme tolerance to the bacterium. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Gram-negative bacteria can produce specific proteinaceous inhibitors to defend themselves against the lytic action of host lysozymes. So far, four different lysozyme inhibitor families have been identified. Here, we report the crystal structure of the Escherichia coli periplasmic lysozyme inhibitor of g-type lysozyme (PliG-Ec) in complex with Atlantic salmon g-type lysozyme (SalG) at a resolution of 0.95 A, which is exceptionally high for a complex of two proteins. The structure reveals for the first time the mechanism of g-type lysozyme inhibition by the PliG family. The latter contains two specific conserved regions that are essential for its inhibitory activity. The inhibitory complex formation is based on a double 'key-lock' mechanism. The first key-lock element is formed by the insertion of two conserved PliG regions into the active site of the lysozyme. The second element is defined by a distinct pocket of PliG accommodating a lysozyme loop. Computational analysis indicates that this pocket represents a suitable site for small molecule binding, which opens an avenue for the development of novel antibacterial agents that suppress the inhibitory activity of PliG. | ||
| - | + | Structural basis of bacterial defense against g-type lysozyme-based innate immunity.,Leysen S, Vanderkelen L, Weeks SD, Michiels CW, Strelkov SV Cell Mol Life Sci. 2012 Oct 21. PMID:23086131<ref>PMID:23086131</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | + | *[[Lysozyme 3D structures|Lysozyme 3D structures]] | |
| - | == | + | == References == |
| - | [[ | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Salmo salar]] | [[Category: Salmo salar]] | ||
| - | [[Category: Leysen, S | + | [[Category: Leysen, S]] |
| - | [[Category: Michiels, C W | + | [[Category: Michiels, C W]] |
| - | [[Category: Strelkov, S V | + | [[Category: Strelkov, S V]] |
| - | [[Category: Vanderkelen, L | + | [[Category: Vanderkelen, L]] |
| - | [[Category: Weeks, S D | + | [[Category: Weeks, S D]] |
[[Category: Hydrolase inhibitor]] | [[Category: Hydrolase inhibitor]] | ||
[[Category: Hydrolase-hydrolase inhibitor complex]] | [[Category: Hydrolase-hydrolase inhibitor complex]] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
Revision as of 01:31, 25 December 2014
Crystal structure of Escherichia coli PliG in complex with Atlantic salmon g-type lysozyme
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