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2avw

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[[Image:2avw.gif|left|200px]]<br /><applet load="2avw" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2avw.gif|left|200px]]
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caption="2avw, resolution 2.0&Aring;" />
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'''Crystal structure of monoclinic form of streptococcus Mac-1'''<br />
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{{Structure
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|PDB= 2avw |SIZE=350|CAPTION= <scene name='initialview01'>2avw</scene>, resolution 2.0&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY=
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|GENE= mac ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198466 Streptococcus pyogenes MGAS315])
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}}
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'''Crystal structure of monoclinic form of streptococcus Mac-1'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2AVW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_mgas315 Streptococcus pyogenes mgas315] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVW OCA].
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2AVW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_mgas315 Streptococcus pyogenes mgas315]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVW OCA].
==Reference==
==Reference==
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Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16472742 16472742]
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Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16472742 16472742]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptococcus pyogenes mgas315]]
[[Category: Streptococcus pyogenes mgas315]]
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[[Category: mac-1]]
[[Category: mac-1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:31:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:54:29 2008''

Revision as of 13:54, 20 March 2008

Image:2avw.gif


PDB ID 2avw

Drag the structure with the mouse to rotate
, resolution 2.0Å
Ligands: and
Gene: mac (Streptococcus pyogenes MGAS315)
Coordinates: save as pdb, mmCIF, xml



Crystal structure of monoclinic form of streptococcus Mac-1


Overview

Group A Streptococcus secretes cysteine proteases named Mac-1 and Mac-2 that mediate host immune evasion by targeting both IgG and Fc receptors. Here, we report the crystal structures of Mac-1 and its catalytically inactive C94A mutant in two different crystal forms. Despite the lack of sequence homology, Mac-1 adopts the canonical papain fold. Alanine mutations at the active site confirmed the critical residues involved in a papain-like catalytic mechanism. Mac-1 forms a symmetric dimer in both crystal forms and displays the unique dimer interface among papain superfamily members. Mutations at the dimer interface resulted in a significant reduction in IgG binding and catalysis, suggesting that the dimer contributes to both IgG specificity and enzyme cooperativity. A tunnel observed at the dimer interface constitutes a target for designing potential Mac-1-specific antimicrobial agents. The structures also offer insight into the functional difference between Mac-1 and Mac-2.

About this Structure

2AVW is a Single protein structure of sequence from Streptococcus pyogenes mgas315. Full crystallographic information is available from OCA.

Reference

Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:16472742

Page seeded by OCA on Thu Mar 20 15:54:29 2008

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