3hhf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "3hhf" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:3hhf.png|left|200px]]
+
==Structure of CrgA regulatory domain, a LysR-type transcriptional regulator from Neisseria meningitidis.==
 +
<StructureSection load='3hhf' size='340' side='right' caption='[[3hhf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3hhf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neisseria_meningitidis_serogroup_b Neisseria meningitidis serogroup b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HHF FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
 +
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 +
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hhg|3hhg]]</td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CrgA, NMB1856 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=491 Neisseria meningitidis serogroup B])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hhf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hhf RCSB], [http://www.ebi.ac.uk/pdbsum/3hhf PDBsum]</span></td></tr>
 +
</table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/3hhf_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
LysR-type transcriptional regulators (LTTRs) form the largest family of bacterial regulators acting as both auto-repressors and activators of target promoters, controlling operons involved in a wide variety of cellular processes. The LTTR, CrgA, from the human pathogen Neisseria meningitidis, is upregulated during bacterial-host cell contact. Here, we report the crystal structures of both regulatory domain and full-length CrgA, the first of a novel subclass of LTTRs that form octameric rings. Non-denaturing mass spectrometry analysis and analytical ultracentrifugation established that the octameric form of CrgA is the predominant species in solution in both the presence and absence of an oligonucleotide encompassing the CrgA-binding sequence. Furthermore, analysis of the isolated CrgA-DNA complex by mass spectrometry showed stabilization of a double octamer species upon DNA binding. Based on the observed structure and the mass spectrometry findings, a model is proposed in which a hexadecameric array of two CrgA oligomers binds to its DNA target site.
-
{{STRUCTURE_3hhf| PDB=3hhf | SCENE= }}
+
The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators.,Sainsbury S, Lane LA, Ren J, Gilbert RJ, Saunders NJ, Robinson CV, Stuart DI, Owens RJ Nucleic Acids Res. 2009 Aug;37(14):4545-58. Epub 2009 May 27. PMID:19474343<ref>PMID:19474343</ref>
-
===Structure of CrgA regulatory domain, a LysR-type transcriptional regulator from Neisseria meningitidis.===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_19474343}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
[[3hhf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neisseria_meningitidis_serogroup_b Neisseria meningitidis serogroup b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HHF OCA].
+
</StructureSection>
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:019474343</ref><references group="xtra"/>
+
[[Category: Neisseria meningitidis serogroup b]]
[[Category: Neisseria meningitidis serogroup b]]
-
[[Category: OPPF, Oxford Protein Production Facility.]]
+
[[Category: OPPF, Oxford Protein Production Facility]]
-
[[Category: Owens, R J.]]
+
[[Category: Owens, R J]]
-
[[Category: Ren, J.]]
+
[[Category: Ren, J]]
-
[[Category: Sainsbury, S.]]
+
[[Category: Sainsbury, S]]
-
[[Category: Stuart, D I.]]
+
[[Category: Stuart, D I]]
[[Category: Dna-binding]]
[[Category: Dna-binding]]
[[Category: Lysr]]
[[Category: Lysr]]

Revision as of 08:41, 8 December 2014

Structure of CrgA regulatory domain, a LysR-type transcriptional regulator from Neisseria meningitidis.

3hhf, resolution 2.30Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3hhf"
Personal tools