Glycosyltransferase

From Proteopedia

(Difference between revisions)

Revision as of 10:44, 20 June 2013

Glycosyltransferase (GT) are enzymes which catalyze the transfer of monosaccharide to alcohol, carbohydrate, protein or inorganic phosphate. Galactosyltransferase (AGT) is a GT which catalyzes the transfer of galactose during the synthesis of polysaccharides. Fucosylglycoprotein 3-AGT (GTB) or histo-blood group ABO system transferase is the basis of the blood ABO system. It involves 3 carbohydrate antigens: A, B and H. Individuals with blood type A, B and AB has glycosyltransferase activitiy which converts antigen H to A or B. Individuals with blood type O lack this activity.

1 3D structures of glycosyltransferase

3D structures of glycosyltransferase

Updated on 20-June-2013

Cyclodextrin GT (CGT)

1cxi, 1cdg, 1cgt, 1cgu - BcCGT – Bacillus circulans

1tcm, 4cgt, 7cgt, 1kck, 1kcl, 1ot1, 1ot2, 1pez, 1pj9, 1v3j, 1v3k, 1cgv, 1cgw - BcCGT (mutant)

1ciu – TtCGT – Thermoanaerobacterium thermosulfurigenes

3bmv, 3bmw – TtCGT (mutant)
1cyg – CGT – Geobacillus stearothermophilus
1d7f - BaCGT (mutant) – Bacillus
1pam - BaCGT

CGT binary complexes

1cxe, 1cxh – BcCGT + substrate

1cxf, 5cgt, 8cgt, 9cgt, 1cxk, 1eo5, 1eo7 - BcCGT (mutant) + substrate

3cgt - BcCGT (mutant) + β-cyclodextrin

1d3c - BcCGT (mutant) + γ-cyclodextrin

6cgt - BcCGT (mutant) + HOXA

2dij, 1dtu - BcCGT (mutant) + inhibitor

1cxl - BcCGT (mutant) + reaction intermediate

2cxg, 1ukq – BcCGT + acarbose-derivative

1uks, 1ukt, 1v3l, 1v3m – BcCGT (mutant) + acarbose-derivative
1ded - BaCGT (mutant) + acarbose
1i75 – BaCGT + deoxyjirimycin
1a47 – TtCGT + inhibitor

Maltose GT

1gju, 1lwh – TmMCG – Thermotoga maritima

1gjw – TmMCG + maltose

1lwj - TmMGT + acarbose

Human GT

1lz0 – hGT A - hGT A catalytic domain + inhibitor

1r7y, 1r81 - hGT A catalytic domain + inhibitor + UDP-derivative

1lz7 – hGT B catalytic domain

1r7u, 1r7x - hGT B catalytic domain + inhibitor

1r80, 1r82 - hGT B catalytic domain + inhibitor + UDP-derivative

1lzi – hGT A catalytic domain + UDP + H antigen acceptor

1lzj – hGT B catalytic domain + UDP + H antigen acceptor

1r7t, 1r7v - hGT ABO(H) + inhibitor

1wt0, 1wsz, 1wt3, 2o1f, 2o1g, 3i0c, 3i0h, 1xz6, 2i7b – hGT ABO(H) (mutant)

1wt1, 1wt2, 2o1h, 3i0d, 3i0i, 2y7a - hGT ABO(H) (mutant) + UDP

3i0e, 3i0j - hGT ABO(H) (mutant) + H-antigen

3i0f, 3i0k - hGT ABO(H) (mutant) + H-antigen + UDP

3i0g, 3i0l - hGT ABO(H) (mutant) + DA + UDP-Gal

Oleandomycin GT

2iya, 2iyf – GT – Strepromyces antibioticus

Hydroquinone GT

2vce – AtGT – Arabidopsis thaliana
2vch, 2vg8 – AtGT + UDP

Flavonoid GT

3hbf – MtGT + UDP + myricetin – Medicago truncatula

3hbj - MtGT + UDP

2pq6 - MtGT

2c1x, 2c1z – GT + UDP – Vitis vinifera

Various GT

1nzd – T4β-GT + UDP-glucose – Phage T4

2nzf, 1nzf - T4β-GT (mutant) + UDP-glucose

2iuy, 2iv3 – SvGT – Stretptomyces viridochromogenes

[3hzs]], 3vmq – SaGT – Staphylococcus aureus
3vmr, 3vms, 3vmt – SaGT + lipid II analog
3mbo – BaGT + UDP + malate – Bacillus anthracis

2jjm - BaGT

3oy7 – GT + GDP-mannose – cholera virus

3pnn – GT – Porphyromonas gingivalis

2q3e, 3q3i, 3q3e – ApGT – Actinobacillus pleuropneumomiae

3q3h – ApGT + UDP-Glc

1iir – AoUDP-GT – Amycolatopsis orientalis

1pn3, 1pnv, 1rrv – AoGT + TDP + vancomycin

2yjn – GT + Dtpd-4-keto-6-deoxy-hexose isomerase – Saccharopolyspora erythraea

3h4i, 3h4t – GT chimera

1cwy – GT – Thermus aquaticus

2iv7, 2iw1 – GT - Escherichia coli

2xci – AaGT – Aquifex aeolicus

2xcu – AaGT + CMP

3ia7 – MeGT Calg4 – Micromonospora echinospora

3iaa – MeGT Calg2 + TDP

3rsc - MeGT Calg2 + TDP + calicheamicin

3otk – GT catalytic domain - mouse
4ayj – BoGT + glucose + galactose + fucose – Bacterioides ovatus
4ayl - BoGT
1g9r - GT (mutant) + UDP-galactose + Mn – Neisseria meningitides

β-1,4-galactosyltransferase (BGT)

1fgx - bBGT catalytic domain – bovine
1pzt - bBGT catalytic domain (mutant)
2fy7 - hBGT catalytic domain (mutant)
3lw6 - BGT catalytic domain (mutant) – Drosophila melanogaster

BGT binary complexes

1fr8, 1o0r - bBGT catalytic domain + UDP-galactose
1tvy, 1tw1 - bBGT catalytic domain (mutant) + UDP-galactose
1tw5 - bBGT catalytic domain (mutant) + chitobiose
1nf5 - bBGT + α-lactalbumin
1o23 - bBGT catalytic domain + UDP-glucose
1oqm - bBGT catalytic domain + UDP-N-acetylgalactosamine
2fya - hBGT catalytic domain (mutant) + Mn
4ee3, 4ee4, 4ee5, 4eea, 4eeg, 4eem, 4eeo - hBGT catalytic domain (mutant) + polysaccharide

BGT ternary complexes

1nhe, 1nkh - bBGT catalytic domain + UDP + α-lactalbumin
1yro - bBGT catalytic domain (mutant) + UDP-galactose + α-lactalbumin
1nwg - bBGT catalytic domain + N-butanoyl-glucoamine + α-lactalbumin
1nmm, 1nqi, 1pzy, 1o7q - bBGT catalytic domain + UDP + GlcNAc
2fyb - hBGT catalytic domain (mutant) + Mn + UDP
2ae7- hBGT catalytic domain + UDP + pentasaccharide
2aec, 2aes, 2agd - hBGT catalytic domain + UDP + GlcNAc + mannose
2fyc - hBGT catalytic domain (mutant) + UDP-galactose + Ca + α-lactalbumin
3ee5 - hBGT catalytic domain (mutant) + UDP + GlcNAc-β1,3-gal-β-naphthalenemethanol
2fyd - hBGT catalytic domain + UDP-GlcNAc + Mn + α-lactalbumin
(mutant) + glucose
2ah9 - hBGT catalytic domain + UDP + chitotriose

α-1,3-galactosyltransferase (AGT)

1g8o, 1fg5, 2jco - bAGT catalytic domain
2jcl – bAGT catalytic domain (mutant)
1vzu, 1vzx, 2i7b - hAGT catalytic domain (mutant)
2i7b - hAGT catalytic domain (mutant)

AGT binary complexes

1g93, 1gx0 - bAGT catalytic domain + UDP-galactose
1k4v - bAGT catalytic domain + UDP
2rj0 - bAGT catalytic domain + UDP + Mn
1vzt, 2jcj - bAGT catalytic domain (mutant) + UDP
2vfz - bAGT catalytic domain (mutant) + UDP-2F-galactose
2vs4, 2vs5, 2vxl, 2vxm, 2vs3 - bAGT catalytic domain (mutant) + UDP-galactose
2vs3 - bAGT catalytic domain + UDP-galactose
1ziz, 1zjp - hAGT catalytic domain + galactose
1zj0 - hAGT catalytic domain + lactose
1zj1 - hAGT catalytic domain + N-acetyl lactosamine
1zj2, 1zj3 - hAGT catalytic domain + trisaccharide
2a8u, 2a8w - hAGT catalytic domain + methyl lactoside

AGT ternary complexes

1gwv - bAGT catalytic domain + UDP + lactose
2jck - bAGT catalytic domain (mutant) + UDP + Mn
2wgz - bAGT catalytic domain + UDP + nitrophenyl-β-galactoside
1o7o - bAGT catalytic domain (mutant) + UDP + lactose
1gx4 - bAGT catalytic domain + UDP + N-acetyl lactosamine
1gww - bAGT catalytic domain + UDP + glucose
1r7u, 1r7x - hAGT catalytic domain + inhibitor + fucose
1r80 - hAGT catalytic domain + UDP + inhibitor + fucose
1r82 - hAGT catalytic domain + UDP-galactose + inhibitor + fucose

Fucosylglycoprotein 3-AGT (GTB)

2pgv, 2pgy, 2riz, 2rj5, 1wsz, 2o1f, 2o1g – hGTB (mutant)
2rit – hGTB
2rix – hGTB + UDP
2o1h – hGT Abo (mutant) + UDP
2riy – hGTB + H-antigen acceptor
1zi4, 1zi5 - hGTB + H-antigen
3i0j – hGT Abo (mutant) + H-antigen
2rj1, 2rj6 - hGTB (mutant) + UDP + H-antigen disaccharide
2rj8 - hGTB + UDP + H-antigen disaccharide
2rj4 - hGTB (mutant) + UDP + ADA
2rj7 - hGTB (mutant) + UDP-galactose + deoxy-acceptor
2rj9 - hGTB + UDP-galactose + deoxy-acceptor
3sx3, 3sx5, 3sx7, 3sx8, 3sxc, 3sxd, 3sxe, 3sxg, 1zhj, 1zjo - hAGT catalytic domain + galactose
1zi1 - hAGT catalytic domain + lactose
1zi3 - hAGT catalytic domain + acetylactosamine

Fucosylgalactoside α N-acetylgalactosaminyltrasferase (GTA)

3ioh – hGTA (mutant)
3ioi - hGTA (mutant) + UDP-galactose derivative
3ioj - hGTA (mutant) + UDP

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

Retrieved from "http://52.214.119.220/wiki/index.php/Glycosyltransferase"

Category: Topic Page

@@ Line 1: / Line 1: @@
 {{STRUCTURE_1cxh|  PDB=1cxh  | SIZE=400| SCENE= |right|CAPTION=Cyclodextrin glycosyltransferase complex with substrate maltoheptaose and Ca+2 ion, [[1cxh]] }}
-'''Glycosyltransferase''' (GT) are enzymes which catalyze the transfer of monosaccharide to alcohol, carbohydrate, protein or inorganic phosphate.
+'''Glycosyltransferase''' (GT) are enzymes which catalyze the transfer of monosaccharide to alcohol, carbohydrate, protein or inorganic phosphate. '''Galactosyltransferase''' (AGT) is a GT which catalyzes  the transfer of galactose during the synthesis of polysaccharides.  Fucosylglycoprotein 3-AGT (GTB) or histo-blood group ABO system transferase is the basis of the blood ABO system.  It involves 3 carbohydrate antigens: A, B and H.  Individuals with blood type A, B and AB has glycosyltransferase activitiy which converts antigen H to A or B.  Individuals with blood type O lack this activity.
 {{TOC limit|limit=2}}
@@ Line 146: / Line 146: @@
 [[3otk]] – GT catalytic domain - mouse<br />
 [[4ayj]] – BoGT + glucose + galactose + fucose – ''Bacterioides ovatus''<br />
-[[4ayl]] - BoGT
+[[4ayl]] - BoGT<br />
+[[1g9r]] - GT (mutant) + UDP-galactose + Mn – Neisseria meningitides
+===β-1,4-galactosyltransferase (BGT)===
+[[1fgx]] - bBGT catalytic domain – bovine<br />
+[[1pzt]] - bBGT catalytic domain (mutant) <br />
+[[2fy7]] - hBGT catalytic domain (mutant) <br />
+[[3lw6]] - BGT catalytic domain (mutant) – Drosophila melanogaster
+===BGT binary complexes===
+[[1fr8]], [[1o0r]] - bBGT catalytic domain + UDP-galactose<br />
+[[1tvy]], [[1tw1]] - bBGT catalytic domain (mutant) + UDP-galactose<br />
+[[1tw5]] - bBGT catalytic domain (mutant) + chitobiose<br />
+[[1nf5]] - bBGT + α-lactalbumin<br />
+[[1o23]] - bBGT catalytic domain + UDP-glucose<br />
+[[1oqm]] - bBGT catalytic domain + UDP-N-acetylgalactosamine<br />
+[[2fya]] - hBGT catalytic domain (mutant) + Mn<br />
+[[4ee3]], [[4ee4]], [[4ee5]], [[4eea]], [[4eeg]], [[4eem]], [[4eeo]] - hBGT catalytic domain (mutant) + polysaccharide<br />
+===BGT ternary complexes===
+[[1nhe]], [[1nkh]] - bBGT catalytic domain + UDP + α-lactalbumin<br />
+[[1yro]] - bBGT catalytic domain (mutant) + UDP-galactose + α-lactalbumin<br />
+[[1nwg]] - bBGT catalytic domain + N-butanoyl-glucoamine + α-lactalbumin<br />
+[[1nmm]], [[1nqi]], [[1pzy]], [[1o7q]] - bBGT catalytic domain + UDP + GlcNAc<br />
+[[2fyb]] - hBGT catalytic domain (mutant) + Mn + UDP<br />
+[[2ae7]]-  hBGT catalytic domain + UDP + pentasaccharide<br />
+[[2aec]], [[2aes]], [[2agd]] - hBGT catalytic domain + UDP + GlcNAc + mannose<br />
+[[2fyc]] - hBGT catalytic domain (mutant) + UDP-galactose + Ca + α-lactalbumin<br />
+[[3ee5]] - hBGT catalytic domain (mutant) + UDP + GlcNAc-β1,3-gal-β-naphthalenemethanol<br />
+[[2fyd]] - hBGT catalytic domain + UDP-GlcNAc + Mn + α-lactalbumin<br /> (mutant) + glucose<br />
+[[2ah9]] - hBGT catalytic domain + UDP + chitotriose<br />
+===α-1,3-galactosyltransferase (AGT)===
+[[1g8o]], [[1fg5]], [[2jco]] - bAGT catalytic domain<br />
+[[2jcl]] – bAGT catalytic domain (mutant) <br />
+[[1vzu]], [[1vzx]], [[2i7b]] - hAGT catalytic domain (mutant)<br />
+[[2i7b]] - hAGT catalytic domain (mutant)
+===AGT binary complexes===
+[[1g93]], [[1gx0]] - bAGT catalytic domain + UDP-galactose<br />
+[[1k4v]] - bAGT catalytic domain + UDP<br />
+[[2rj0]] - bAGT catalytic domain + UDP + Mn<br />
+[[1vzt]], [[2jcj]] - bAGT catalytic domain (mutant) + UDP<br />
+[[2vfz]] - bAGT catalytic domain (mutant) + UDP-2F-galactose<br />
+[[2vs4]], [[2vs5]], [[2vxl]], [[2vxm]], [[2vs3]] - bAGT catalytic domain (mutant) + UDP-galactose<br />
+[[2vs3]] - bAGT catalytic domain  + UDP-galactose<br />
+[[1ziz]], [[1zjp]] - hAGT catalytic domain + galactose<br />
+[[1zj0]] - hAGT catalytic domain + lactose<br />
+[[1zj1]] - hAGT catalytic domain + N-acetyl lactosamine<br />
+[[1zj2]], [[1zj3]] - hAGT catalytic domain + trisaccharide<br />
+[[2a8u]], [[2a8w]] - hAGT catalytic domain + methyl lactoside<br />
+===AGT ternary complexes===
+[[1gwv]] - bAGT catalytic domain + UDP + lactose<br />
+[[2jck]] - bAGT catalytic domain (mutant) + UDP + Mn<br />
+[[2wgz]] - bAGT catalytic domain + UDP + nitrophenyl-β-galactoside<br />
+[[1o7o]] - bAGT catalytic domain (mutant) + UDP + lactose<br />
+[[1gx4]] - bAGT catalytic domain + UDP + N-acetyl lactosamine<br />
+[[1gww]] - bAGT catalytic domain + UDP + glucose<br />
+[[1r7u]], [[1r7x]] - hAGT catalytic domain + inhibitor + fucose<br />
+[[1r80]] - hAGT catalytic domain + UDP + inhibitor + fucose<br />
+[[1r82]] - hAGT catalytic domain + UDP-galactose + inhibitor + fucose<br />
+===Fucosylglycoprotein 3-AGT (GTB)===
+[[2pgv]], [[2pgy]], [[2riz]], [[2rj5]], [[1wsz]], [[2o1f]], [[2o1g]] – hGTB (mutant) <br />
+[[2rit ]]– hGTB<br />
+[[2rix]] – hGTB + UDP<br />
+[[2o1h]] – hGT Abo (mutant) + UDP<br />
+[[2riy]] – hGTB + H-antigen acceptor<br />
+[[1zi4]], [[1zi5]] - hGTB + H-antigen<br />
+[[3i0j]] – hGT Abo (mutant) + H-antigen<br />
+[[2rj1]], [[2rj6]] - hGTB (mutant) + UDP + H-antigen disaccharide<br />
+[[2rj8]] - hGTB  + UDP + H-antigen disaccharide<br />
+[[2rj4]] - hGTB (mutant) + UDP + ADA<br />
+[[2rj7]] - hGTB (mutant) + UDP-galactose + deoxy-acceptor<br />
+[[2rj9]] - hGTB  + UDP-galactose + deoxy-acceptor<br />
+[[3sx3]], [[3sx5]], [[3sx7]], [[3sx8]], [[3sxc]], [[3sxd]], [[3sxe]], [[3sxg]], [[1zhj]], [[1zjo]] - hAGT catalytic domain + galactose<br />
+[[1zi1]] - hAGT catalytic domain + lactose<br />
+[[1zi3]] - hAGT catalytic domain + acetylactosamine
+===Fucosylgalactoside α N-acetylgalactosaminyltrasferase (GTA)===
+[[3ioh]] – hGTA (mutant) <br />
+[[3ioi]] - hGTA (mutant) + UDP-galactose derivative<br />
+[[3ioj]] - hGTA (mutant) + UDP
 [[Category:Topic Page]]