2b3j
From Proteopedia
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| - | [[Image:2b3j.gif|left|200px]] | + | [[Image:2b3j.gif|left|200px]] |
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| - | '''Crystal Structure of Staphylococcus aureus tRNA Adenosine Deaminase, TadA, in Complex with RNA''' | + | {{Structure |
| + | |PDB= 2b3j |SIZE=350|CAPTION= <scene name='initialview01'>2b3j</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= TADA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 Staphylococcus aureus subsp. aureus Mu50]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Staphylococcus aureus tRNA Adenosine Deaminase, TadA, in Complex with RNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2B3J is a [ | + | 2B3J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3J OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA., Losey HC, Ruthenburg AJ, Verdine GL, Nat Struct Mol Biol. 2006 Feb;13(2):153-9. Epub 2006 Jan 15. PMID:[http:// | + | Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA., Losey HC, Ruthenburg AJ, Verdine GL, Nat Struct Mol Biol. 2006 Feb;13(2):153-9. Epub 2006 Jan 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16415880 16415880] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus subsp. aureus mu50]] | [[Category: Staphylococcus aureus subsp. aureus mu50]] | ||
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[[Category: rna stem-loop]] | [[Category: rna stem-loop]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:57:05 2008'' |
Revision as of 13:57, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Ligands: | and | ||||||
| Gene: | TADA (Staphylococcus aureus subsp. aureus Mu50) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Staphylococcus aureus tRNA Adenosine Deaminase, TadA, in Complex with RNA
Overview
Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different arginine codons in mRNA. In addition, inosine is also introduced at the wobble position of multiple eukaryotic tRNAs. The genes encoding these deaminases are essential in bacteria and yeast, demonstrating the importance of their biological activity. Here we report the crystallization and structure determination to 2.0 A of Staphylococcus aureus TadA bound to the anticodon stem-loop of tRNA(Arg2) bearing nebularine, a non-hydrolyzable adenosine analog, at the wobble position. The cocrystal structure reveals the basis for both sequence and structure specificity in the interactions of TadA with RNA, and it additionally provides insight into the active site architecture that promotes efficient hydrolytic deamination.
About this Structure
2B3J is a Single protein structure of sequence from Staphylococcus aureus subsp. aureus mu50. Full crystallographic information is available from OCA.
Reference
Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA., Losey HC, Ruthenburg AJ, Verdine GL, Nat Struct Mol Biol. 2006 Feb;13(2):153-9. Epub 2006 Jan 15. PMID:16415880
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