3fbv

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{{Large structure}}
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==Crystal structure of the oligomer formed by the kinase-ribonuclease domain of Ire1==
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{{STRUCTURE_3fbv| PDB=3fbv | SCENE= }}
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<StructureSection load='3fbv' size='340' side='right' caption='[[3fbv]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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===Crystal structure of the oligomer formed by the kinase-ribonuclease domain of Ire1===
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== Structural highlights ==
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{{ABSTRACT_PUBMED_19079236}}
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<table><tr><td colspan='2'>[[3fbv]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FBV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APJ:N~2~-1H-BENZIMIDAZOL-5-YL-N~4~-(3-CYCLOPROPYL-1H-PYRAZOL-5-YL)PYRIMIDINE-2,4-DIAMINE'>APJ</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IRE1, ERN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fbv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fbv RCSB], [http://www.ebi.ac.uk/pdbsum/3fbv PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Aberrant folding of proteins in the endoplasmic reticulum activates the bifunctional transmembrane kinase/endoribonuclease Ire1. Ire1 excises an intron from HAC1 messenger RNA in yeasts and Xbp1 messenger RNA in metozoans encoding homologous transcription factors. This non-conventional mRNA splicing event initiates the unfolded protein response, a transcriptional program that relieves the endoplasmic reticulum stress. Here we show that oligomerization is central to Ire1 function and is an intrinsic attribute of its cytosolic domains. We obtained the 3.2-A crystal structure of the oligomer of the Ire1 cytosolic domains in complex with a kinase inhibitor that acts as a potent activator of the Ire1 RNase. The structure reveals a rod-shaped assembly that has no known precedence among kinases. This assembly positions the kinase domain for trans-autophosphorylation, orders the RNase domain, and creates an interaction surface for binding of the mRNA substrate. Activation of Ire1 through oligomerization expands the mechanistic repertoire of kinase-based signalling receptors.
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==About this Structure==
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The unfolded protein response signals through high-order assembly of Ire1.,Korennykh AV, Egea PF, Korostelev AA, Finer-Moore J, Zhang C, Shokat KM, Stroud RM, Walter P Nature. 2009 Feb 5;457(7230):687-93. Epub 2008 Dec 14. PMID:19079236<ref>PMID:19079236</ref>
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[[3fbv]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FBV OCA].
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
==See Also==
==See Also==
*[[Ire1|Ire1]]
*[[Ire1|Ire1]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019079236</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Egea, P F.]]
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[[Category: Egea, P F]]
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[[Category: Finer-Moore, J.]]
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[[Category: Finer-Moore, J]]
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[[Category: Korennykh, A V.]]
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[[Category: Korennykh, A V]]
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[[Category: Korostelev, A A.]]
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[[Category: Korostelev, A A]]
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[[Category: Shokat, K M.]]
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[[Category: Shokat, K M]]
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[[Category: Stroud, R M.]]
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[[Category: Stroud, R M]]
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[[Category: Walter, P.]]
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[[Category: Walter, P]]
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[[Category: Zhang, C.]]
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[[Category: Zhang, C]]
[[Category: Aminopyrazole]]
[[Category: Aminopyrazole]]
[[Category: Apy29]]
[[Category: Apy29]]

Revision as of 14:11, 17 December 2014

Crystal structure of the oligomer formed by the kinase-ribonuclease domain of Ire1

3fbv, resolution 3.20Å

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