3k55
From Proteopedia
(Difference between revisions)
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| - | + | ==Structure of beta hairpin deletion mutant of beta toxin from Staphylococcus aureus== | |
| - | + | <StructureSection load='3k55' size='340' side='right' caption='[[3k55]], [[Resolution|resolution]] 3.35Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3k55]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K55 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K55 FirstGlance]. <br> | |
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hlb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sphingomyelin_phosphodiesterase Sphingomyelin phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.12 3.1.4.12] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k55 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k55 RCSB], [http://www.ebi.ac.uk/pdbsum/3k55 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The 3.35 A resolution crystal structure of a mutant form of the staphylococcal sphingomyelinase beta toxin in which a conserved hydrophobic beta-hairpin has been deleted is reported. It is shown that this mutation induces domain swapping of a C-terminal beta-strand, leading to the formation of dimers linked by a conformationally flexible hinge region. Eight dimers are seen in the asymmetric unit, exhibiting a broad spectrum of conformations trapped in place by intermolecular contacts within the crystal lattice. Furthermore, the 16 monomers within each asymmetric unit exhibit a remarkable heterogeneity in thermal factors, which can be accounted for by the varying degrees to which each monomer interacts with other molecules in the crystal. This structure provides a unique example of the challenges associated with crystallographic study of flexible proteins. | ||
| - | + | Structure of a mutant beta toxin from Staphylococcus aureus reveals domain swapping and conformational flexibility.,Kruse AC, Huseby MJ, Shi K, Digre J, Ohlendorf DH, Earhart CA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt, 4):438-41. Epub 2011 Mar 24. PMID:21505235<ref>PMID:21505235</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Hemolysin|Hemolysin]] | *[[Hemolysin|Hemolysin]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Sphingomyelin phosphodiesterase]] | [[Category: Sphingomyelin phosphodiesterase]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
| - | [[Category: Digre, J | + | [[Category: Digre, J]] |
| - | [[Category: Earhart, C A | + | [[Category: Earhart, C A]] |
| - | [[Category: Huseby, M | + | [[Category: Huseby, M]] |
| - | [[Category: Kruse, A C | + | [[Category: Kruse, A C]] |
| - | [[Category: Ohlendorf, D H | + | [[Category: Ohlendorf, D H]] |
| - | [[Category: Shi, K | + | [[Category: Shi, K]] |
[[Category: Beta toxin]] | [[Category: Beta toxin]] | ||
[[Category: Domain swapping]] | [[Category: Domain swapping]] | ||
Revision as of 06:04, 18 December 2014
Structure of beta hairpin deletion mutant of beta toxin from Staphylococcus aureus
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