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3j01

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{{Large structure}}
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==Structure of the ribosome-SecYE complex in the membrane environment==
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{{STRUCTURE_3j01| PDB=3j01 | SCENE= }}
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<StructureSection load='3j01' size='340' side='right' caption='[[3j01]], [[Resolution|resolution]] 7.10&Aring;' scene=''>
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===Structure of the ribosome-SecYE complex in the membrane environment===
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== Structural highlights ==
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{{ABSTRACT_PUBMED_21499241}}
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<table><tr><td colspan='2'>[[3j01]] is a 33 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecol5 Ecol5], [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Escherichia_coli_dh1 Escherichia coli dh1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J01 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j00|3j00]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j01 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j01 RCSB], [http://www.ebi.ac.uk/pdbsum/3j01 PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The ubiquitous SecY-Sec61 complex translocates nascent secretory proteins across cellular membranes and integrates membrane proteins into lipid bilayers. Several structures of mostly detergent-solubilized Sec complexes have been reported. Here we present a single-particle cryo-EM structure of the SecYEG complex in a membrane environment, bound to a translating ribosome, at subnanometer resolution. Using the SecYEG complex reconstituted in a so-called Nanodisc, we could trace the nascent polypeptide chain from the peptidyltransferase center into the membrane. The reconstruction allowed for the identification of ribosome-lipid interactions. The rRNA helix 59 (H59) directly contacts the lipid surface and appears to modulate the membrane in immediate vicinity to the proposed lateral gate of the protein-conducting channel (PCC). On the basis of our map and molecular dynamics simulations, we present a model of a signal anchor-gated PCC in the membrane.
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==About this Structure==
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Cryo-EM structure of the ribosome-SecYE complex in the membrane environment.,Frauenfeld J, Gumbart J, Sluis EO, Funes S, Gartmann M, Beatrix B, Mielke T, Berninghausen O, Becker T, Schulten K, Beckmann R Nat Struct Mol Biol. 2011 May;18(5):614-21. Epub 2011 Apr 17. PMID:21499241<ref>PMID:21499241</ref>
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[[3j01]] is a 33 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_536 Escherichia coli 536], [http://en.wikipedia.org/wiki/Escherichia_coli_dh1 Escherichia coli dh1] and [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J01 OCA].
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==See Also==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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*[[Ribosomal protein L1|Ribosomal protein L1]]
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</div>
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*[[Ribosomal protein L2|Ribosomal protein L2]]
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*[[Ribosomal protein L3|Ribosomal protein L3]]
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*[[Ribosomal protein L4|Ribosomal protein L4]]
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*[[Ribosomal protein L5|Ribosomal protein L5]]
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*[[Ribosomal protein L6|Ribosomal protein L6]]
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*[[Ribosomal protein L9|Ribosomal protein L9]]
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==Reference==
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==See Also==
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<ref group="xtra">PMID:021499241</ref><references group="xtra"/>
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*[[Preprotein translocase|Preprotein translocase]]
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[[Category: Escherichia coli 536]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Ecol5]]
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[[Category: Ecoli]]
[[Category: Escherichia coli dh1]]
[[Category: Escherichia coli dh1]]
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[[Category: Escherichia coli k-12]]
 
[[Category: Beatrix, B.]]
[[Category: Beatrix, B.]]
[[Category: Becker, T.]]
[[Category: Becker, T.]]

Revision as of 06:43, 30 July 2014

Structure of the ribosome-SecYE complex in the membrane environment

3j01, resolution 7.10Å

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