3j01

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{Large structure}}
+
==Structure of the ribosome-SecYE complex in the membrane environment==
-
{{STRUCTURE_3j01| PDB=3j01 | SCENE= }}
+
<StructureSection load='3j01' size='340' side='right' caption='[[3j01]], [[Resolution|resolution]] 7.10&Aring;' scene=''>
-
===Structure of the ribosome-SecYE complex in the membrane environment===
+
== Structural highlights ==
-
{{ABSTRACT_PUBMED_21499241}}
+
<table><tr><td colspan='2'>[[3j01]] is a 33 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecol5 Ecol5], [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Escherichia_coli_dh1 Escherichia coli dh1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J01 FirstGlance]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j00|3j00]]</td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j01 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j01 RCSB], [http://www.ebi.ac.uk/pdbsum/3j01 PDBsum]</span></td></tr>
 +
<table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The ubiquitous SecY-Sec61 complex translocates nascent secretory proteins across cellular membranes and integrates membrane proteins into lipid bilayers. Several structures of mostly detergent-solubilized Sec complexes have been reported. Here we present a single-particle cryo-EM structure of the SecYEG complex in a membrane environment, bound to a translating ribosome, at subnanometer resolution. Using the SecYEG complex reconstituted in a so-called Nanodisc, we could trace the nascent polypeptide chain from the peptidyltransferase center into the membrane. The reconstruction allowed for the identification of ribosome-lipid interactions. The rRNA helix 59 (H59) directly contacts the lipid surface and appears to modulate the membrane in immediate vicinity to the proposed lateral gate of the protein-conducting channel (PCC). On the basis of our map and molecular dynamics simulations, we present a model of a signal anchor-gated PCC in the membrane.
-
==About this Structure==
+
Cryo-EM structure of the ribosome-SecYE complex in the membrane environment.,Frauenfeld J, Gumbart J, Sluis EO, Funes S, Gartmann M, Beatrix B, Mielke T, Berninghausen O, Becker T, Schulten K, Beckmann R Nat Struct Mol Biol. 2011 May;18(5):614-21. Epub 2011 Apr 17. PMID:21499241<ref>PMID:21499241</ref>
-
[[3j01]] is a 33 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_536 Escherichia coli 536], [http://en.wikipedia.org/wiki/Escherichia_coli_dh1 Escherichia coli dh1] and [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J01 OCA].
+
-
==See Also==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
*[[Ribosomal protein L1|Ribosomal protein L1]]
+
</div>
-
*[[Ribosomal protein L2|Ribosomal protein L2]]
+
-
*[[Ribosomal protein L3|Ribosomal protein L3]]
+
-
*[[Ribosomal protein L4|Ribosomal protein L4]]
+
-
*[[Ribosomal protein L5|Ribosomal protein L5]]
+
-
*[[Ribosomal protein L6|Ribosomal protein L6]]
+
-
*[[Ribosomal protein L9|Ribosomal protein L9]]
+
-
==Reference==
+
==See Also==
-
<ref group="xtra">PMID:021499241</ref><references group="xtra"/>
+
*[[Preprotein translocase|Preprotein translocase]]
-
[[Category: Escherichia coli 536]]
+
*[[Ribosome 3D structures|Ribosome 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Ecol5]]
 +
[[Category: Ecoli]]
[[Category: Escherichia coli dh1]]
[[Category: Escherichia coli dh1]]
-
[[Category: Escherichia coli k-12]]
 
[[Category: Beatrix, B.]]
[[Category: Beatrix, B.]]
[[Category: Becker, T.]]
[[Category: Becker, T.]]

Revision as of 06:43, 30 July 2014

Structure of the ribosome-SecYE complex in the membrane environment

3j01, resolution 7.10Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j01"
Views
Personal tools
Navigation
Toolbox