3mtt
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal structure of iSH2 domain of human p85beta, Northeast Structural Genomics Consortium Target HR5531C== | |
| - | + | <StructureSection load='3mtt' size='340' side='right' caption='[[3mtt]], [[Resolution|resolution]] 3.30Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3mtt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MTT FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PIK3R2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mtt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mtt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mtt RCSB], [http://www.ebi.ac.uk/pdbsum/3mtt PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mt/3mtt_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Phosphatidylinositol 3-kinase (PI3K) proteins actively trigger signaling pathways leading to cell growth, proliferation and survival. These proteins have multiple isoforms and consist of a catalytic p110 subunit and a regulatory p85 subunit. The iSH2 domain of the p85beta isoform has been implicated in the binding of nonstructural protein 1 (NS1) of influenza A viruses. Here, the crystal structure of human p85beta iSH2 determined to 3.3 A resolution is reported. The structure reveals that this domain mainly consists of a coiled-coil motif. Comparison with the published structure of the bovine p85beta iSH2 domain bound to the influenza A virus nonstructural protein 1 indicates that little or no structural change occurs upon complex formation. By comparing this human p85beta iSH2 structure with the bovine p85beta iSH2 domain, which shares 99% sequence identity, and by comparing the multiple conformations observed within the asymmetric unit of the bovine iSH2 structure, it was found that this coiled-coil domain exhibits a certain degree of conformational variability or `plasticity' in the interhelical turn region. It is speculated that this plasticity of p85beta iSH2 may play a role in regulating its functional and molecular-recognition properties. | ||
| - | + | Structure of the iSH2 domain of human phosphatidylinositol 3-kinase p85beta subunit reveals conformational plasticity in the interhelical turn region.,Schauder C, Ma LC, Krug RM, Montelione GT, Guan R Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt, 12):1567-71. Epub 2010 Nov 16. PMID:21139197<ref>PMID:21139197</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Phosphoinositide 3-Kinases|Phosphoinositide 3-Kinases]] | *[[Phosphoinositide 3-Kinases|Phosphoinositide 3-Kinases]] | ||
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | < | + | </StructureSection> |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Guan, R | + | [[Category: Guan, R]] |
| - | [[Category: Krug, R M | + | [[Category: Krug, R M]] |
| - | [[Category: Ma, L C | + | [[Category: Ma, L C]] |
| - | [[Category: Montelione, G T | + | [[Category: Montelione, G T]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: Schauder, C | + | [[Category: Schauder, C]] |
[[Category: Inter-sh2 domain]] | [[Category: Inter-sh2 domain]] | ||
[[Category: Nesg]] | [[Category: Nesg]] | ||
| - | [[Category: Northeast structural genomics consortium]] | ||
[[Category: Pi3-kinase subunit p85-beta]] | [[Category: Pi3-kinase subunit p85-beta]] | ||
[[Category: Protein binding]] | [[Category: Protein binding]] | ||
| - | [[Category: Protein structure initiative | + | [[Category: PSI, Protein structure initiative]] |
| - | + | ||
[[Category: Signaling protein]] | [[Category: Signaling protein]] | ||
| - | [[Category: Structural genomic]] | ||
Revision as of 15:17, 18 December 2014
Crystal structure of iSH2 domain of human p85beta, Northeast Structural Genomics Consortium Target HR5531C
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