1f4k
From Proteopedia
(Difference between revisions)
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| - | + | ==CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX== | |
| - | + | <StructureSection load='1f4k' size='340' side='right' caption='[[1f4k]], [[Resolution|resolution]] 2.50Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1f4k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F4K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F4K FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bm9|1bm9]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f4k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1f4k RCSB], [http://www.ebi.ac.uk/pdbsum/1f4k PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The coordinated termination of DNA replication is an important step in the life cycle of bacteria with circular chromosomes, but has only been defined at a molecular level in two systems to date. Here we report the structure of an engineered replication terminator protein (RTP) of Bacillus subtilis in complex with a 21 base pair DNA by X-ray crystallography at 2.5 A resolution. We also use NMR spectroscopic titration techniques. This work reveals a novel DNA interaction involving a dimeric 'winged helix' domain protein that differs from predictions. While the two recognition helices of RTP are in close contact with the B-form DNA major grooves, the 'wings' and N-termini of RTP do not form intimate contacts with the DNA. This structure provides insight into the molecular basis of polar replication fork arrest based on a model of cooperative binding and differential binding affinities of RTP to the two adjacent binding sites in the complete terminator. | ||
| - | + | Structure of the RTP-DNA complex and the mechanism of polar replication fork arrest.,Wilce JA, Vivian JP, Hastings AF, Otting G, Folmer RH, Duggin IG, Wake RG, Wilce MC Nat Struct Biol. 2001 Mar;8(3):206-10. PMID:11224562<ref>PMID:11224562</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Bacterial Replication Termination|Bacterial Replication Termination]] | *[[Bacterial Replication Termination|Bacterial Replication Termination]] | ||
| - | *[[RTP and Tus|RTP and Tus]] | ||
*[[Replication Termination Protein|Replication Termination Protein]] | *[[Replication Termination Protein|Replication Termination Protein]] | ||
*[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]] | *[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]] | ||
*[[User:Chloe Paul/Replication Terminator Protein|User:Chloe Paul/Replication Terminator Protein]] | *[[User:Chloe Paul/Replication Terminator Protein|User:Chloe Paul/Replication Terminator Protein]] | ||
*[[User:Wayne Decatur/Tomato aspermy virus protein 2b Suppression of RNA Silencing|User:Wayne Decatur/Tomato aspermy virus protein 2b Suppression of RNA Silencing]] | *[[User:Wayne Decatur/Tomato aspermy virus protein 2b Suppression of RNA Silencing|User:Wayne Decatur/Tomato aspermy virus protein 2b Suppression of RNA Silencing]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
| - | [[Category: Duggin, I G | + | [[Category: Duggin, I G]] |
| - | [[Category: Folmer, R H.A | + | [[Category: Folmer, R H.A]] |
| - | [[Category: Hastings, A F | + | [[Category: Hastings, A F]] |
| - | [[Category: Otting, G | + | [[Category: Otting, G]] |
| - | [[Category: Vivian, J P | + | [[Category: Vivian, J P]] |
| - | [[Category: Wake, R G | + | [[Category: Wake, R G]] |
| - | [[Category: Wilce, J A | + | [[Category: Wilce, J A]] |
| - | [[Category: Wilce, M C.J | + | [[Category: Wilce, M C.J]] |
[[Category: Fork arrest mechanism]] | [[Category: Fork arrest mechanism]] | ||
[[Category: Replication and termination]] | [[Category: Replication and termination]] | ||
[[Category: Replication-dna complex]] | [[Category: Replication-dna complex]] | ||
[[Category: Winged-helix protein-dna complex]] | [[Category: Winged-helix protein-dna complex]] | ||
Revision as of 13:51, 17 December 2014
CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX
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