2bep

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[[Image:2bep.gif|left|200px]]<br /><applet load="2bep" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2bep.gif|left|200px]]
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caption="2bep, resolution 1.80&Aring;" />
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'''CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K'''<br />
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{{Structure
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|PDB= 2bep |SIZE=350|CAPTION= <scene name='initialview01'>2bep</scene>, resolution 1.80&Aring;
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|SITE= <scene name='pdbsite=AC1:Bme+Binding+Site+For+Chain+A'>AC1</scene>
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|LIGAND= <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19]
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2BEP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Known structural/functional Site: <scene name='pdbsite=AC1:Bme+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEP OCA].
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2BEP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEP OCA].
==Reference==
==Reference==
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SUMO modification of the ubiquitin-conjugating enzyme E2-25K., Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK, Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15723079 15723079]
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SUMO modification of the ubiquitin-conjugating enzyme E2-25K., Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK, Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15723079 15723079]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: protein degradation]]
[[Category: protein degradation]]
[[Category: spine]]
[[Category: spine]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
[[Category: structural proteomics in europe]]
[[Category: structural proteomics in europe]]
[[Category: ubiquitin]]
[[Category: ubiquitin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:37:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:00:58 2008''

Revision as of 14:00, 20 March 2008

Image:2bep.gif


PDB ID 2bep

Drag the structure with the mouse to rotate
, resolution 1.80Å
Sites:
Ligands:
Activity: Ubiquitin--protein ligase, with EC number 6.3.2.19
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K


Overview

Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets.

About this Structure

2BEP is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

SUMO modification of the ubiquitin-conjugating enzyme E2-25K., Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK, Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. PMID:15723079

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