2bfn

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[[Image:2bfn.gif|left|200px]]<br /><applet load="2bfn" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2bfn.gif|left|200px]]
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caption="2bfn, resolution 1.600&Aring;" />
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'''THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.'''<br />
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{{Structure
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|PDB= 2bfn |SIZE=350|CAPTION= <scene name='initialview01'>2bfn</scene>, resolution 1.600&Aring;
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|SITE= <scene name='pdbsite=AC1:D2p+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Ca+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Ca+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:Ca+Binding+Site+For+Chain+A'>AC5</scene>
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=D2P:(2S)-2,3-DICHLOROPROPAN-1-OL'>D2P</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2BFN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=D2P:'>D2P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:D2p+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Ca+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Ca+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:Ca+Binding+Site+For+Chain+A'>AC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFN OCA].
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2BFN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFN OCA].
==Reference==
==Reference==
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Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-Ray crystallography and microcalorimetry., Monincova M, Prokop Z, Vevodova J, Nagata Y, Damborsky J, Appl Environ Microbiol. 2007 Mar;73(6):2005-8. Epub 2007 Jan 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17259360 17259360]
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Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-Ray crystallography and microcalorimetry., Monincova M, Prokop Z, Vevodova J, Nagata Y, Damborsky J, Appl Environ Microbiol. 2007 Mar;73(6):2005-8. Epub 2007 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17259360 17259360]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Banas, P.]]
[[Category: Banas, P.]]
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[[Category: hydrolase]]
[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:37:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:01:19 2008''

Revision as of 14:01, 20 March 2008

Image:2bfn.gif


PDB ID 2bfn

Drag the structure with the mouse to rotate
, resolution 1.600Å
Sites: , , , and
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.


Overview

1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP, since the reaction cannot be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (k(cat) = 0.005 s(-1)) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for the development of a new biocatalyst toward TCP by protein engineering. Microcalorimetry is proposed to be a universal method for the detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering novel biocatalysts using the scaffolds of proteins with promiscuous activities.

About this Structure

2BFN is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-Ray crystallography and microcalorimetry., Monincova M, Prokop Z, Vevodova J, Nagata Y, Damborsky J, Appl Environ Microbiol. 2007 Mar;73(6):2005-8. Epub 2007 Jan 26. PMID:17259360

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