1i25
From Proteopedia
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| - | + | ==Three dimensional solution structure of huwentoxin-II by 2D 1H-NMR== | |
| - | + | <StructureSection load='1i25' size='340' side='right' caption='[[1i25]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1i25]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haplopelma_schmidti Haplopelma schmidti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I25 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i25 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i25 RCSB], [http://www.ebi.ac.uk/pdbsum/1i25 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The three-dimensional structure of huwentoxin-II (HWTX-II), an insecticidal peptide purified from the venom of spider Selenocosmia huwena with a unique disulfide bond linkage as I-III, II-V, and IV-VI, has been determined using 2D (1)H-NMR. The resulting structure of HWTX-II contains two beta-turns (C4-S7 and K24-W27) and a double-stranded antiparallel beta-sheet (W27-C29 and C34-K36). Although the C-terminal double-stranded beta-sheet cross-linked by two disulfide bonds (II-V and IV-VI in HWTX-II, II-V and III-VI in the ICK molecules) is conserved both in HWTX-II and the ICK molecules, the structure of HWTX-II is unexpected absence of the cystine knot because of its unique disulfide linkage. It suggests that HWTX-II adopts a novel scaffold different from the ICK motif that is adopted by all other spider toxin structures elucidated thus far. Furthermore, the structure of HWTX-II, which conforms to the disulfide-directed beta-hairpin (DDH) motif, not only supports the hypothesis that the ICK is a minor elaboration of the more ancestral DDH motif but also suggests that HWTX-II may have evolved from the same structural ancestor. | ||
| - | + | The structure of spider toxin huwentoxin-II with unique disulfide linkage: evidence for structural evolution.,Shu Q, Lu SY, Gu XC, Liang SP Protein Sci. 2002 Feb;11(2):245-52. PMID:11790834<ref>PMID:11790834</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[NMR Ensembles of Models|NMR Ensembles of Models]] | *[[NMR Ensembles of Models|NMR Ensembles of Models]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Haplopelma schmidti]] | [[Category: Haplopelma schmidti]] | ||
| - | [[Category: Gu, X C | + | [[Category: Gu, X C]] |
| - | [[Category: Liang, S P | + | [[Category: Liang, S P]] |
| - | [[Category: Lu, S Y | + | [[Category: Lu, S Y]] |
| - | [[Category: Shu, Q | + | [[Category: Shu, Q]] |
[[Category: Disulfide bond]] | [[Category: Disulfide bond]] | ||
[[Category: Insecticidal toxin]] | [[Category: Insecticidal toxin]] | ||
[[Category: Neurotoxin]] | [[Category: Neurotoxin]] | ||
[[Category: Toxin]] | [[Category: Toxin]] | ||
Revision as of 05:38, 22 December 2014
Three dimensional solution structure of huwentoxin-II by 2D 1H-NMR
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