1fjg
From Proteopedia
(Difference between revisions)
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| - | + | ==STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTICS STREPTOMYCIN, SPECTINOMYCIN, AND PAROMOMYCIN== | |
| - | + | <StructureSection load='1fjg' size='340' side='right' caption='[[1fjg]], [[Resolution|resolution]] 3.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1fjg]] is a 22 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The October 2000 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Ribosome'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2000_10 10.2210/rcsb_pdb/mom_2000_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FJG FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=SCM:SPECTINOMYCIN'>SCM</scene>, <scene name='pdbligand=SRY:STREPTOMYCIN'>SRY</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1qd7|1qd7]], [[1fjf|1fjf]]</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fjg RCSB], [http://www.ebi.ac.uk/pdbsum/1fjg PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/1fjg_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The 30S ribosomal subunit has two primary functions in protein synthesis. It discriminates against aminoacyl transfer RNAs that do not match the codon of messenger RNA, thereby ensuring accuracy in translation of the genetic message in a process called decoding. Also, it works with the 50S subunit to move the tRNAs and associated mRNA by precisely one codon, in a process called translocation. Here we describe the functional implications of the high-resolution 30S crystal structure presented in the accompanying paper, and infer details of the interactions between the 30S subunit and its tRNA and mRNA ligands. We also describe the crystal structure of the 30S subunit complexed with the antibiotics paromomycin, streptomycin and spectinomycin, which interfere with decoding and translocation. This work reveals the structural basis for the action of these antibiotics, and leads to a model for the role of the universally conserved 16S RNA residues A1492 and A1493 in the decoding process. | ||
| - | + | Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics.,Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Wimberly BT, Ramakrishnan V Nature. 2000 Sep 21;407(6802):340-8. PMID:11014183<ref>PMID:11014183</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Nobel Prizes for 3D Molecular Structure|Nobel Prizes for 3D Molecular Structure]] | *[[Nobel Prizes for 3D Molecular Structure|Nobel Prizes for 3D Molecular Structure]] | ||
| - | *[[Ribosomal protein S10|Ribosomal protein S10]] | ||
| - | *[[Ribosomal protein S11|Ribosomal protein S11]] | ||
| - | *[[Ribosomal protein S12|Ribosomal protein S12]] | ||
| - | *[[Ribosomal protein S13|Ribosomal protein S13]] | ||
| - | *[[Ribosomal protein S14|Ribosomal protein S14]] | ||
| - | *[[Ribosomal protein S15|Ribosomal protein S15]] | ||
| - | *[[Ribosomal protein S16|Ribosomal protein S16]] | ||
| - | *[[Ribosomal protein S17|Ribosomal protein S17]] | ||
| - | *[[Ribosomal protein S18|Ribosomal protein S18]] | ||
| - | *[[Ribosomal protein S19|Ribosomal protein S19]] | ||
| - | *[[Ribosomal protein S2|Ribosomal protein S2]] | ||
| - | *[[Ribosomal protein S20|Ribosomal protein S20]] | ||
| - | *[[Ribosomal protein S3|Ribosomal protein S3]] | ||
| - | *[[Ribosomal protein S4|Ribosomal protein S4]] | ||
| - | *[[Ribosomal protein S5|Ribosomal protein S5]] | ||
| - | *[[Ribosomal protein S6|Ribosomal protein S6]] | ||
| - | *[[Ribosomal protein S7|Ribosomal protein S7]] | ||
| - | *[[Ribosomal protein S8|Ribosomal protein S8]] | ||
| - | *[[Ribosomal protein S9|Ribosomal protein S9]] | ||
*[[Ribosomal protein THX|Ribosomal protein THX]] | *[[Ribosomal protein THX|Ribosomal protein THX]] | ||
*[[Ribosome|Ribosome]] | *[[Ribosome|Ribosome]] | ||
| - | + | *[[Ribosome 3D structures|Ribosome 3D structures]] | |
| - | == | + | == References == |
| - | < | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: RCSB PDB Molecule of the Month]] | [[Category: RCSB PDB Molecule of the Month]] | ||
[[Category: Ribosome]] | [[Category: Ribosome]] | ||
Revision as of 17:11, 29 September 2014
STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTICS STREPTOMYCIN, SPECTINOMYCIN, AND PAROMOMYCIN
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