2bjv

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[[Image:2bjv.gif|left|200px]]<br /><applet load="2bjv" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2bjv.gif|left|200px]]
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caption="2bjv, resolution 1.70&Aring;" />
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'''CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT'''<br />
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{{Structure
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|PDB= 2bjv |SIZE=350|CAPTION= <scene name='initialview01'>2bjv</scene>, resolution 1.70&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2BJV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJV OCA].
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2BJV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJV OCA].
==Reference==
==Reference==
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Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15790859 15790859]
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Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15790859 15790859]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: transcription regulation]]
[[Category: transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:38:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:02:54 2008''

Revision as of 14:02, 20 March 2008

Image:2bjv.gif


PDB ID 2bjv

Drag the structure with the mouse to rotate
, resolution 1.70Å
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT


Overview

Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.

About this Structure

2BJV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:15790859

Page seeded by OCA on Thu Mar 20 16:02:54 2008

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