1ema
From Proteopedia
(Difference between revisions)
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| - | + | ==GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA== | |
| - | === | + | <StructureSection load='1ema' size='340' side='right' caption='[[1ema]], [[Resolution|resolution]] 1.90Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1ema]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. The June 2003 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Green Fluorescent Protein (GFP)'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2003_6 10.2210/rcsb_pdb/mom_2003_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EMA FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ema FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ema OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ema RCSB], [http://www.ebi.ac.uk/pdbsum/1ema PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/1ema_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria has generated intense interest as a marker for gene expression and localization of gene products. The chromophore, resulting from the spontaneous cyclization and oxidation of the sequence -Ser65 (or Thr65)-Tyr66-Gly67-, requires the native protein fold for both formation and fluorescence emission. The structure of Thr65 GFP has been determined at 1.9 angstrom resolution. The protein fold consists of an 11-stranded beta barrel with a coaxial helix, with the chromophore forming from the central helix. Directed mutagenesis of one residue adjacent to the chromophore, Thr203, to Tyr or His results in significantly red-shifted excitation and emission maxima. | ||
| - | + | Crystal structure of the Aequorea victoria green fluorescent protein.,Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075<ref>PMID:8703075</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
| - | *[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]] | ||
| - | *[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]] | ||
*[[Gfp vc2|Gfp vc2]] | *[[Gfp vc2|Gfp vc2]] | ||
*[[Green Fluorescent Protein|Green Fluorescent Protein]] | *[[Green Fluorescent Protein|Green Fluorescent Protein]] | ||
*[[Green Fluorescent Protein: Research Tool|Green Fluorescent Protein: Research Tool]] | *[[Green Fluorescent Protein: Research Tool|Green Fluorescent Protein: Research Tool]] | ||
| - | *[[ | + | *[[Proteopedia.org/wiki/index.php/User:Joel L. Sussman/GFP Test Page|Proteopedia.org/wiki/index.php/User:Joel L. Sussman/GFP Test Page]] |
| - | *[[User: | + | *[[Proteopedia.org/wiki/workshop/GFP Background|Proteopedia.org/wiki/workshop/GFP Background]] |
| + | *[[User:Joel L. Sussman/GFP Test Page|User:Joel L. Sussman/GFP Test Page]] | ||
*[[User:Lynmarie K Thompson/2009Chem490|User:Lynmarie K Thompson/2009Chem490]] | *[[User:Lynmarie K Thompson/2009Chem490|User:Lynmarie K Thompson/2009Chem490]] | ||
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | < | + | </StructureSection> |
[[Category: Aequorea victoria]] | [[Category: Aequorea victoria]] | ||
[[Category: RCSB PDB Molecule of the Month]] | [[Category: RCSB PDB Molecule of the Month]] | ||
Revision as of 05:33, 3 October 2014
GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA
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