2bmh
From Proteopedia
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| - | [[Image:2bmh.gif|left|200px]] | + | [[Image:2bmh.gif|left|200px]] |
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| - | '''MODELING PROTEIN-SUBSTRATE INTERACTIONS IN THE HEME DOMAIN OF CYTOCHROME P450BM-3''' | + | {{Structure |
| + | |PDB= 2bmh |SIZE=350|CAPTION= <scene name='initialview01'>2bmh</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''MODELING PROTEIN-SUBSTRATE INTERACTIONS IN THE HEME DOMAIN OF CYTOCHROME P450BM-3''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BMH is a [ | + | 2BMH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BMH OCA]. |
==Reference== | ==Reference== | ||
| - | Modeling protein-substrate interactions in the heme domain of cytochrome P450(BM-3)., Li H, Poulos TL, Acta Crystallogr D Biol Crystallogr. 1995 Jan 1;51(Pt 1):21-32. PMID:[http:// | + | Modeling protein-substrate interactions in the heme domain of cytochrome P450(BM-3)., Li H, Poulos TL, Acta Crystallogr D Biol Crystallogr. 1995 Jan 1;51(Pt 1):21-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299332 15299332] |
[[Category: Bacillus megaterium]] | [[Category: Bacillus megaterium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: oxidoreductase(oxygenase)]] | [[Category: oxidoreductase(oxygenase)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:03:51 2008'' |
Revision as of 14:03, 20 March 2008
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| , resolution 2.0Å | |||||||
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| Ligands: | |||||||
| Activity: | Unspecific monooxygenase, with EC number 1.14.14.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
MODELING PROTEIN-SUBSTRATE INTERACTIONS IN THE HEME DOMAIN OF CYTOCHROME P450BM-3
Overview
The crystal structure of heme domain of the fatty acid monooxygenase, cytochrome P450(BM-3), consisting of residues 1-455 has been independently solved to R = 0.18 at 2.0 A. The crystal form used, space group P2(1) with two molecules per asymmetric unit, is isomorphous with that form with residues 1-471 first described by Boddupalli et al. [Boddupalli, Hasemann, Ravinchandran, Lu, Goldsmith, Deisenhofer & Peterson (1992). Proc. Natl Acad. Sci. USA, 89, 5567-5571] and used by Ravichandran, Boddupalli, Hasemann, Peterson & Deisenhofer [(1993). Science, 261, 731-736] to determine the crystal structure. The substrate-access channel consists of a large, hydrophobic cleft that appears to be the most likely route taken by fatty acid substrates. Attempts to soak crystals in mother liquor containing a variety of fatty acid substrates yielded featureless difference Fouriers even though fatty acid substrates are known to bind with dissociation constants in the micro M range. Modeling substrate-enzyme interactions reveals few contacts between the enzyme and substrate. More detailed modeling was carried out by subjecting both molecules in the asymmetric unit to extensive energy minimization. These studies reveal that the heme-domain active-site cleft can undergo a large conformational change that closes the access channel thereby providing enhanced protein-substrate interactions. These conformational changes are prevented from occurring by intermolecular contacts in the crystal lattice which lock the protein in the 'open' conformation.
About this Structure
2BMH is a Single protein structure of sequence from Bacillus megaterium. Full crystallographic information is available from OCA.
Reference
Modeling protein-substrate interactions in the heme domain of cytochrome P450(BM-3)., Li H, Poulos TL, Acta Crystallogr D Biol Crystallogr. 1995 Jan 1;51(Pt 1):21-32. PMID:15299332
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