3ois
From Proteopedia
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| - | + | ==Crystal Structure Xylellain, a cysteine protease from Xylella fastidiosa== | |
| - | + | <StructureSection load='3ois' size='340' side='right' caption='[[3ois]], [[Resolution|resolution]] 1.65Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3ois]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Xylella_fastidiosa Xylella fastidiosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OIS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OIS FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">XF_0156 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2371 Xylella fastidiosa])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ois FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ois OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ois RCSB], [http://www.ebi.ac.uk/pdbsum/3ois PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Xylella fastidiosa is responsible for a wide range of economically important plant diseases. We report here the crystal structure and kinetic data of Xylellain, the first cysteine protease characterized from the genome of the pathogenic X. fastidiosa strain 9a5c. Xylellain has a papain-family fold, and part of the N-terminal sequence blocks the enzyme active site, thereby mediating protein activity. One novel feature identified in the structure is the presence of a ribonucleotide bound outside the active site. We show that this ribonucleotide plays an important regulatory role in Xylellain enzyme kinetics, possibly functioning as a physiological mediator. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: Xylellain and Xylellainbind by X-ray crystallography (View interaction). | ||
| - | + | The crystal structure of the cysteine protease Xylellain from Xylella fastidiosa reveals an intriguing activation mechanism.,Leite NR, Faro AR, Dotta MA, Faim LM, Gianotti A, Silva FH, Oliva G, Thiemann OH FEBS Lett. 2013 Feb 14;587(4):339-44. doi: 10.1016/j.febslet.2013.01.009. Epub, 2013 Jan 17. PMID:23333295<ref>PMID:23333295</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Xylella fastidiosa]] | [[Category: Xylella fastidiosa]] | ||
| - | [[Category: Faro, A R | + | [[Category: Faro, A R]] |
| - | [[Category: Leite, N R | + | [[Category: Leite, N R]] |
| - | [[Category: Oliva, G | + | [[Category: Oliva, G]] |
| - | [[Category: Oliva, M A.V | + | [[Category: Oliva, M A.V]] |
| - | [[Category: Thiemann, O H | + | [[Category: Thiemann, O H]] |
[[Category: Alpha and beta]] | [[Category: Alpha and beta]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
Revision as of 08:32, 18 December 2014
Crystal Structure Xylellain, a cysteine protease from Xylella fastidiosa
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