2byu
From Proteopedia
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| - | [[Image:2byu.gif|left|200px]] | + | [[Image:2byu.gif|left|200px]] |
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| - | '''NEGATIVE STAIN EM RECONSTRUCTION OF M.TUBERCULOSIS ACR1(HSP 16.3) FITTED WITH WHEAT SHSP DIMER''' | + | {{Structure |
| + | |PDB= 2byu |SIZE=350|CAPTION= <scene name='initialview01'>2byu</scene>, resolution 16.5Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NEGATIVE STAIN EM RECONSTRUCTION OF M.TUBERCULOSIS ACR1(HSP 16.3) FITTED WITH WHEAT SHSP DIMER''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BYU is a [ | + | 2BYU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BYU OCA]. |
==Reference== | ==Reference== | ||
| - | Dodecameric structure of the small heat shock protein Acr1 from Mycobacterium tuberculosis., Kennaway CK, Benesch JL, Gohlke U, Wang L, Robinson CV, Orlova EV, Saibil HR, Keep NH, J Biol Chem. 2005 Sep 30;280(39):33419-25. Epub 2005 Jul 26. PMID:[http:// | + | Dodecameric structure of the small heat shock protein Acr1 from Mycobacterium tuberculosis., Kennaway CK, Benesch JL, Gohlke U, Wang L, Robinson CV, Orlova EV, Saibil HR, Keep NH, J Biol Chem. 2005 Sep 30;280(39):33419-25. Epub 2005 Jul 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16046399 16046399] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Triticum aestivum]] | [[Category: Triticum aestivum]] | ||
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[[Category: small heat shock protein]] | [[Category: small heat shock protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:08:32 2008'' |
Revision as of 14:08, 20 March 2008
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| , resolution 16.5Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
NEGATIVE STAIN EM RECONSTRUCTION OF M.TUBERCULOSIS ACR1(HSP 16.3) FITTED WITH WHEAT SHSP DIMER
Overview
Small heat shock proteins are a ubiquitous and diverse family of stress proteins that have in common an alpha-crystallin domain. Mycobacterium tuberculosis has two small heat shock proteins, Acr1 (alpha-crystallin-related protein 1, or Hsp16.3/16-kDa antigen) and Acr2 (HrpA), both of which are highly expressed under different stress conditions. Small heat shock proteins form large oligomeric assemblies and are commonly polydisperse. Nanoelectrospray mass spectrometry showed that Acr2 formed a range of oligomers composed of dimers and tetramers, whereas Acr1 was a dodecamer. Electron microscopy of Acr2 showed a variety of particle sizes. Using three-dimensional analysis of negative stain electron microscope images, we have shown that Acr1 forms a tetrahedral assembly with 12 polypeptide chains. The atomic structure of a related alpha-crystallin domain dimer was docked into the density to build a molecular structure of the dodecameric Acr1 complex. Along with the differential regulation of these two proteins, the differences in their quaternary structures demonstrated here supports their distinct functional roles.
About this Structure
2BYU is a Single protein structure of sequence from Triticum aestivum. Full crystallographic information is available from OCA.
Reference
Dodecameric structure of the small heat shock protein Acr1 from Mycobacterium tuberculosis., Kennaway CK, Benesch JL, Gohlke U, Wang L, Robinson CV, Orlova EV, Saibil HR, Keep NH, J Biol Chem. 2005 Sep 30;280(39):33419-25. Epub 2005 Jul 26. PMID:16046399
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