2bz2
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2bz2.gif|left|200px]] | + | [[Image:2bz2.gif|left|200px]] |
| - | + | ||
| - | '''SOLUTION STRUCTURE OF NELF E RRM''' | + | {{Structure |
| + | |PDB= 2bz2 |SIZE=350|CAPTION= <scene name='initialview01'>2bz2</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''SOLUTION STRUCTURE OF NELF E RRM''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2BZ2 is a [ | + | 2BZ2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ2 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural studies on the RNA-recognition motif of NELF E, a cellular negative transcription elongation factor involved in the regulation of HIV transcription., Rao JN, Neumann L, Wenzel S, Schweimer K, Rosch P, Wohrl BM, Biochem J. 2006 Dec 15;400(3):449-56. PMID:[http:// | + | Structural studies on the RNA-recognition motif of NELF E, a cellular negative transcription elongation factor involved in the regulation of HIV transcription., Rao JN, Neumann L, Wenzel S, Schweimer K, Rosch P, Wohrl BM, Biochem J. 2006 Dec 15;400(3):449-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16898873 16898873] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 32: | Line 41: | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:08:38 2008'' |
Revision as of 14:08, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF NELF E RRM
Contents |
Overview
The elongation of transcription of HIV RNA at the TAR (transactivation-response element) is highly regulated by positive and negative factors. The cellular negative transcription elongation factor NELF (negative elongation factor) was suggested to be involved in transcriptional regulation of HIV-1 (HIV type 1) by binding to the stem of the viral TAR RNA which is synthesized by cellular RNA polymerase II at the viral long terminal repeat. NELF is a heterotetrameric protein consisting of NELF A, B, C or the splice variant D, and E. In the present study, we determined the solution structure of the RRM (RNA-recognition motif) of the RNA-binding subunit NELF E and studied its interaction with the viral TAR RNA. Our results show that the separately expressed recombinant NELF E RRM has alpha-helical and beta-strand elements adopting a betaalphabetabetaalphabeta fold and is able to bind to TAR RNA. Fluorescence equilibrium titrations with fluorescently labelled double- and single-stranded oligoribonucleotides representing the TAR RNA stem imply that NELF E RRM binds to the single-stranded TAR RNAs with K(d) values in the low-micromolar range.
Disease
Known diseases associated with this structure: Blood group, Radin OMIM:[609017], Blood group, Scianna system OMIM:[609017]
About this Structure
2BZ2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural studies on the RNA-recognition motif of NELF E, a cellular negative transcription elongation factor involved in the regulation of HIV transcription., Rao JN, Neumann L, Wenzel S, Schweimer K, Rosch P, Wohrl BM, Biochem J. 2006 Dec 15;400(3):449-56. PMID:16898873
Page seeded by OCA on Thu Mar 20 16:08:38 2008
