2c37

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2c37.gif|left|200px]]<br /><applet load="2c37" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:2c37.gif|left|200px]]
-
caption="2c37, resolution 2.80&Aring;" />
+
 
-
'''RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH U8 RNA'''<br />
+
{{Structure
 +
|PDB= 2c37 |SIZE=350|CAPTION= <scene name='initialview01'>2c37</scene>, resolution 2.80&Aring;
 +
|SITE= <scene name='pdbsite=AC1:U5p+Binding+Site+For+Chain+X'>AC1</scene>
 +
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=U5P:URIDINE-5'-MONOPHOSPHATE'>U5P</scene>, <scene name='pdbligand=U5P:URIDINE-5'-MONOPHOSPHATE'>U5P</scene> and <scene name='pdbligand=RP5:RIBOSE-5-PHOSPHATE, PYRANOSE FORM'>RP5</scene>
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH U8 RNA'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
2C37 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=U5P:'>U5P</scene>, <scene name='pdbligand=U5P:'>U5P</scene> and <scene name='pdbligand=RP5:'>RP5</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 2C37 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb86_1.html Exosomes]]. Known structural/functional Site: <scene name='pdbsite=AC1:U5p+Binding+Site+For+Chain+X'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C37 OCA].
+
2C37 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. The following page contains interesting information on the relation of 2C37 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb86_1.html Exosomes]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C37 OCA].
==Reference==
==Reference==
-
Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core., Lorentzen E, Conti E, Mol Cell. 2005 Nov 11;20(3):473-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16285928 16285928]
+
Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core., Lorentzen E, Conti E, Mol Cell. 2005 Nov 11;20(3):473-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16285928 16285928]
[[Category: Exosomes]]
[[Category: Exosomes]]
[[Category: Protein complex]]
[[Category: Protein complex]]
Line 30: Line 39:
[[Category: rrp42]]
[[Category: rrp42]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:44:22 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:10:11 2008''

Revision as of 14:10, 20 March 2008

Image:2c37.gif


PDB ID 2c37

Drag the structure with the mouse to rotate
, resolution 2.80Å
Sites:
Ligands: , , , and
Coordinates: save as pdb, mmCIF, xml



RNASE PH CORE OF THE ARCHAEAL EXOSOME IN COMPLEX WITH U8 RNA


Overview

The exosome is a macromolecular complex that plays fundamental roles in the biogenesis and turnover of a large number of RNA species. Here we report the crystal structures of the Rrp41-Rrp42 core complex of the S. solfataricus exosome bound to short single-stranded RNAs and to ADP. The RNA binding cleft recognizes four nucleotides in a sequence-unspecific manner, mainly by electrostatic interactions with the phosphate groups. Interactions at the 2' hydroxyls of the sugars provide specificity for RNA over DNA. The structures show both the bound substrate and the cleaved product of the reaction, suggesting a catalytic mechanism for the 3'-5' phosphorolytic activity of the exosome.

About this Structure

2C37 is a Protein complex structure of sequences from Sulfolobus solfataricus. The following page contains interesting information on the relation of 2C37 with [Exosomes]. Full crystallographic information is available from OCA.

Reference

Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core., Lorentzen E, Conti E, Mol Cell. 2005 Nov 11;20(3):473-81. PMID:16285928

Page seeded by OCA on Thu Mar 20 16:10:11 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2c37"
Personal tools