2c8o

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[[Image:2c8o.gif|left|200px]]<br /><applet load="2c8o" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2c8o.gif|left|200px]]
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caption="2c8o, resolution 1.50&Aring;" />
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'''LYSOZYME (1SEC) AND UV LASR EXCITED FLUORESCENCE'''<br />
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{{Structure
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|PDB= 2c8o |SIZE=350|CAPTION= <scene name='initialview01'>2c8o</scene>, resolution 1.50&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]
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|GENE=
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}}
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'''LYSOZYME (1SEC) AND UV LASR EXCITED FLUORESCENCE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2C8O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8O OCA].
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2C8O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8O OCA].
==Reference==
==Reference==
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UV laser-excited fluorescence as a tool for the visualization of protein crystals mounted in loops., Vernede X, Lavault B, Ohana J, Nurizzo D, Joly J, Jacquamet L, Felisaz F, Cipriani F, Bourgeois D, Acta Crystallogr D Biol Crystallogr. 2006 Mar;62(Pt 3):253-61. Epub 2006, Feb 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16510972 16510972]
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UV laser-excited fluorescence as a tool for the visualization of protein crystals mounted in loops., Vernede X, Lavault B, Ohana J, Nurizzo D, Joly J, Jacquamet L, Felisaz F, Cipriani F, Bourgeois D, Acta Crystallogr D Biol Crystallogr. 2006 Mar;62(Pt 3):253-61. Epub 2006, Feb 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16510972 16510972]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: visualisation]]
[[Category: visualisation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:46:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:12:19 2008''

Revision as of 14:12, 20 March 2008

Image:2c8o.gif


PDB ID 2c8o

Drag the structure with the mouse to rotate
, resolution 1.50Å
Activity: Lysozyme, with EC number 3.2.1.17
Coordinates: save as pdb, mmCIF, xml



LYSOZYME (1SEC) AND UV LASR EXCITED FLUORESCENCE


Overview

Structural proteomics has promoted the rapid development of automated protein structure determination using X-ray crystallography. Robotics are now routinely used along the pipeline from genes to protein structures. However, a bottleneck still remains. At synchrotron beamlines, the success rate of automated sample alignment along the X-ray beam is limited by difficulties in visualization of protein crystals, especially when they are small and embedded in mother liquor. Despite considerable improvement in optical microscopes, the use of visible light transmitted or reflected by the sample may result in poor or misleading contrast. Here, the endogenous fluorescence from aromatic amino acids has been used to identify even tiny or weakly fluorescent crystals with a high success rate. The use of a compact laser at 266 nm in combination with non-fluorescent sample holders provides an efficient solution to collect high-contrast fluorescence images in a few milliseconds and using standard camera optics. The best image quality was obtained with direct illumination through a viewing system coaxial with the UV beam. Crystallographic data suggest that the employed UV exposures do not generate detectable structural damage.

About this Structure

2C8O is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

UV laser-excited fluorescence as a tool for the visualization of protein crystals mounted in loops., Vernede X, Lavault B, Ohana J, Nurizzo D, Joly J, Jacquamet L, Felisaz F, Cipriani F, Bourgeois D, Acta Crystallogr D Biol Crystallogr. 2006 Mar;62(Pt 3):253-61. Epub 2006, Feb 22. PMID:16510972

Page seeded by OCA on Thu Mar 20 16:12:19 2008

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