2cfp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2cfp.gif|left|200px]]<br /><applet load="2cfp" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:2cfp.gif|left|200px]]
-
caption="2cfp, resolution 3.30&Aring;" />
+
 
-
'''SUGAR FREE LACTOSE PERMEASE AT ACIDIC PH'''<br />
+
{{Structure
 +
|PDB= 2cfp |SIZE=350|CAPTION= <scene name='initialview01'>2cfp</scene>, resolution 3.30&Aring;
 +
|SITE= <scene name='pdbsite=AC2:Hg+Binding+Site+For+Chain+A'>AC2</scene>
 +
|LIGAND= <scene name='pdbligand=HG:MERCURY (II) ION'>HG</scene>
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''SUGAR FREE LACTOSE PERMEASE AT ACIDIC PH'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
2CFP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=HG:'>HG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC2:Hg+Binding+Site+For+Chain+A'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFP OCA].
+
2CFP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFP OCA].
==Reference==
==Reference==
-
Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY., Mirza O, Guan L, Verner G, Iwata S, Kaback HR, EMBO J. 2006 Mar 22;25(6):1177-83. Epub 2006 Mar 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16525509 16525509]
+
Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY., Mirza O, Guan L, Verner G, Iwata S, Kaback HR, EMBO J. 2006 Mar 22;25(6):1177-83. Epub 2006 Mar 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16525509 16525509]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 26: Line 35:
[[Category: transport mechanism]]
[[Category: transport mechanism]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:48:06 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:14:50 2008''

Revision as of 14:14, 20 March 2008

Image:2cfp.gif


PDB ID 2cfp

Drag the structure with the mouse to rotate
, resolution 3.30Å
Sites:
Ligands:
Coordinates: save as pdb, mmCIF, xml



SUGAR FREE LACTOSE PERMEASE AT ACIDIC PH


Overview

Cation-coupled active transport is an essential cellular process found ubiquitously in all living organisms. Here, we present two novel ligand-free X-ray structures of the lactose permease (LacY) of Escherichia coli determined at acidic and neutral pH, and propose a model for the mechanism of coupling between lactose and H+ translocation. No sugar-binding site is observed in the absence of ligand, and deprotonation of the key residue Glu269 is associated with ligand binding. Thus, substrate induces formation of the sugar-binding site, as well as the initial step in H+ transduction.

About this Structure

2CFP is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY., Mirza O, Guan L, Verner G, Iwata S, Kaback HR, EMBO J. 2006 Mar 22;25(6):1177-83. Epub 2006 Mar 9. PMID:16525509

Page seeded by OCA on Thu Mar 20 16:14:50 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2cfp"
Personal tools