1odg
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 1ODG is a | + | 1ODG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ODG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:50:18 2007'' |
Revision as of 12:45, 5 November 2007
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VERY-SHORT-PATCH DNA REPAIR ENDONUCLEASE BOUND TO ITS REACTION PRODUCT SITE
Overview
Very-short-patch repair (Vsr) enzymes occur in a variety of bacteria, where they initiate nucleotide excision repair of G:T mismatches arising, by deamination of 5-methyl-cytosines in specific regulatory sequences. We, have now determined the structure of the archetypal dcm-Vsr endonuclease, from Escherichia coli bound to the cleaved authentic, hemi-deaminated/hemi-methylated dcm sequence 5'-C-OH-3', 5'-p-T-p-A-p-G-p-G-3'/3'-G-p-G-p-T-p(Me5)C-p-C formed by self-assembly of, a 12mer oligonucleotide into a continuous nicked DNA superhelix. The, structure reveals the presence of a Hoogsteen base pair within the, deaminated recognition sequence and the substantial distortions of the DNA, that accompany Vsr binding to product sites.
About this Structure
1ODG is a Protein complex structure of sequences from Escherichia coli with ZN as ligand. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Escherichia coli dcm very-short-patch DNA repair endonuclease bound to its reaction product-site in a DNA superhelix., Bunting KA, Roe SM, Headley A, Brown T, Savva R, Pearl LH, Nucleic Acids Res. 2003 Mar 15;31(6):1633-9. PMID:12626704
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